I have to perform Western Blot on FFPE tissue and the first part is the extraction.
First I did deparaffination, then I added the extraction buffer (20 mM Tris-HCl pH 8, 2% SDS) and I incubated for 20 minutes at 99°C and for 2h at 80°C.
This is the result with and without pellet. Can I understand by eye if the proteins are in here? Is there some clue that makes me understand that the lysis didn't occur correctly? Or do I have to wait until staining with ponceau S?
Someone that did something similar could tell me if this is the right apperance?
Thanks for your help