What is the best treatment to block autophagy in C2C12 cell line?

I read that NH4CL and chloroquine treatments can be used to block autophagic flux. Are there any other acceptable treatments?

02 September 2020 4,252 3 View

How to generate heterozygous knockouts using CRISPR Cas9 in iPSCs?

Hi all. I'm trying to create a heterozygous deletion (55bp) mutation in iPSCs to model malignant tranformation. I'm using two sgRNAs in a plasmid based system (puromycin selection). I obtained ~20...

18 May 2020 5,389 1 View

5x5 crosstabs table on spss, iteration keeps going up and no results , anyone know why?

Hey all, So im trying to run 5X5 crosstabs with fisher exact test on SPSS, got about 1000~ lines in the file. Im seeing the iteration number keeps going up (1700 currently), but nothing happens...

07 December 2018 5,602 4 View

PVA freezing/thawing cycles - when is it better to "pause"?

I am currently working with PVA using the physical polymerization method, of multiple freezing/thawing (F/T) cycles. Since I've been getting opposite opinions regarding this question, I wanted to...

25 February 2017 2,797 1 View

How to oxidize Nitric oxide?

20 June 2016 2,008 9 View

Where can I purchase Silica nanoparticles?

In the past I worked with silica nanoparticles bought from "Nissan Chemicals", Product No. ST-40, see link for other types.In the company site I can't find that silica anymore.How can I get that...

17 August 2014 6,289 10 View

What are the best ADHD diagnostic methods, given that my goal is to associate between risky driving and sub-impairments (or sub-dimensions) of ADHD?

Hi, I am studying the links between ADHD sub-dimensions and the different types of risky driving. What are the best ADHD diagnostic methods, given that my goal is to associate between risky...

01 January 1970 2,805 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

No title

02 March 2021 932 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View