What is the best treatment to block autophagy in C2C12 cell line?

I read that NH4CL and chloroquine treatments can be used to block autophagic flux. Are there any other acceptable treatments?

02 September 2020 4,252 3 View

How to generate heterozygous knockouts using CRISPR Cas9 in iPSCs?

Hi all. I'm trying to create a heterozygous deletion (55bp) mutation in iPSCs to model malignant tranformation. I'm using two sgRNAs in a plasmid based system (puromycin selection). I obtained ~20...

18 May 2020 5,389 1 View

Are there guidelines for conducting a meta analysis and scoping review on case reports?

Currently, I can only find case reports on the rare condition I am planning to conduct a meta-analysis on. I will be evaluating outcomes and efficacy of treatments. Are there any known guidelines...

24 February 2020 5,742 3 View

5x5 crosstabs table on spss, iteration keeps going up and no results , anyone know why?

Hey all, So im trying to run 5X5 crosstabs with fisher exact test on SPSS, got about 1000~ lines in the file. Im seeing the iteration number keeps going up (1700 currently), but nothing happens...

07 December 2018 5,602 4 View

PVA freezing/thawing cycles - when is it better to "pause"?

I am currently working with PVA using the physical polymerization method, of multiple freezing/thawing (F/T) cycles. Since I've been getting opposite opinions regarding this question, I wanted to...

25 February 2017 2,797 1 View

How to oxidize Nitric oxide?

20 June 2016 2,008 9 View

Where can I purchase Silica nanoparticles?

In the past I worked with silica nanoparticles bought from "Nissan Chemicals", Product No. ST-40, see link for other types.In the company site I can't find that silica anymore.How can I get that...

17 August 2014 6,289 10 View

What are the best ADHD diagnostic methods, given that my goal is to associate between risky driving and sub-impairments (or sub-dimensions) of ADHD?

Hi, I am studying the links between ADHD sub-dimensions and the different types of risky driving. What are the best ADHD diagnostic methods, given that my goal is to associate between risky...

01 January 1970 2,805 1 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

How long can you store Matrigel-coated Transwell inserts?

Hi, everyone! I was planning to do an invasion assay today and thus coated my inserts yesterday, but unfortunately, due to certain circumstances, I cannot proceed. The next time that I can do the...

09 February 2021 8,771 3 View

Meniscus formation matrigel?

Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...

09 February 2021 6,979 3 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View

Spontaneous metastasis of nsg mice reason for early metastasis?

Hello. I have injected breast cancer cells with matrigel in 4th inguinal mammary fat pad of nsg mice. i was not expecting the cells to metastasize before 2 weeks but when I imaged them on day...

31 January 2021 2,817 2 View

How to do immunostaining with 3D culture (tissue embedded in matrigel)?

I dissected tissue from mice then embedded it in matrigel for culture. For analysis, I plan to do immunostaining with some cell markers. But the regular IF protocol did not work. Matrigel would...

11 January 2021 6,961 3 View

Des anybody have a straight forward protocol for invasion assay using colon cancer stem cells? Is there necessary to dilute the Matrigel?

I am working on colon cancer stem cells to see the impact of combinational therapy.

23 December 2020 9,830 3 View

Why the oral cancer cell line from metastatic tongue origin is showing less aggressive property?

I am working with oral cancer cell line SCC090 (ATCC). As per ATCC data sheet it is originated from metastatic tongue. It's primary site is not mentioned. Due to the origination from metastatic...

08 December 2020 5,674 3 View

How do you fix organoids for IHC staining and H&E sections??

I work with ovarian cancer cell line organoids embedded in matrigel, they grow well the A2780 cells, but once I start using 10% formaldehyde to fix them they disintegrate...does anybody have a...

29 November 2020 1,900 1 View

Whats the Alternate for cell recovery solution from matrigel ??

Hello, my work involves growing cells in the matrigel and for recovering cells from matrigel i usually use cell recovery solution from corning. As now i am curious to know if theres an alternate...

20 November 2020 1,148 3 View