Any Protocols for Growing/Differentiating C2C12 Myoblast Cells on Cover Slip in a 6 Well Plate?

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Any statistical methods for comparing Betas scores in mixed linear model?

hi there, I'm looking for assessing the importance of different features within a mixed linear model, and to be able to tell statistically (i.e. to be able to talk about significant difference)...

07 May 2024 8,177 6 View

Any good mouse mir tissue databases out there?

I’m working on a 3’UTR tissue optimizer software, and I’m looking for a mouse mir expression database. The few I could find were lacking in most tissues. While the human databases are very...

31 January 2023 6,869 0 View

Can someone help in identifying this species?

Eastern Mediterranean Sessile Soft

05 July 2022 1,647 0 View

Problems with running RNA and mRNA on agarose gel?

I've started working in a new lab, i think there is some problem with equipment or loading dye or something. They have been having this problem before i got here and have been ignoring it, but...

10 November 2021 7,566 5 View

How to calculate sample size from MDC and SD using Gpower?

Hey everyone! I wonder what is the right way to calculate my sample size for two unrelated groups comparison if I am to base my effect size on the MDC and SD of my primary outcome. In g power...

07 September 2021 2,690 1 View

How to remove aluminium metal contact without harming the underlying metal oxide layer ?

I have a MOS structure- Al/HfO2/Si. I want to remove the aluminium metal contact without harming/etching the underlying hafnium oxide film. Can Nitric acid do that since hafnium is unaffected by...

23 March 2021 4,474 4 View

What is the best treatment to block autophagy in C2C12 cell line?

I read that NH4CL and chloroquine treatments can be used to block autophagic flux. Are there any other acceptable treatments?

02 September 2020 4,388 3 View

How to generate heterozygous knockouts using CRISPR Cas9 in iPSCs?

Hi all. I'm trying to create a heterozygous deletion (55bp) mutation in iPSCs to model malignant tranformation. I'm using two sgRNAs in a plasmid based system (puromycin selection). I obtained ~20...

18 May 2020 5,531 1 View

Any studies exploring multiple-cue discrimination learning in one valance in Drosophila or elsewhere?

I am trying to find examples of research on invertebrates, especially drosophila, examining discrimination learning or differential associative learning. Specifically, I am looking for work in in...

13 May 2020 6,311 0 View

Are there guidelines for conducting a meta analysis and scoping review on case reports?

Currently, I can only find case reports on the rare condition I am planning to conduct a meta-analysis on. I will be evaluating outcomes and efficacy of treatments. Are there any known guidelines...

24 February 2020 5,893 3 View

Are these iPSC colonies?

I’m currently reprogramming my fibroblast cells using Simplicon RNA OKSG-cMyc TagRFP Kit and after 13 days, these colony/aggregates started to show up in all of my wells. I have 6 wells, 2 wells...

07 July 2024 9,034 2 View

Breaking organoids to single-cell suspension while passaging?

I'm currently working with mouse uterine organoids and I've seen that many authors perform organoid passaging using mechanical or enzymatic methods to dissolve the Matrigel while breaking the...

01 July 2024 381 2 View

What are these clumps in my transfected PBMCs?

Hello everyone. I cultured isolated PBMCs in Erythroid Expansion Medium for two weeks and then performed electroporation in my expanded PBMCs in order to reprogram them. The first 24h after the...

22 June 2024 346 4 View

Is it possible to form a cell monolayer on top of Matrigel within an invasion assay?

I am currently using transwell inserts with the addition of 50 uL of matrigel for an invasion assay with various osteosarcoma cell lines. I have tried various dilutions of matrigel and all result...

17 June 2024 8,695 0 View

Why is iPSC in bad condition?

I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...

12 June 2024 6,087 0 View

What's wrong with my iPSC cell morphology?

This shape gradually spreads. Even if you install new cells again, it will change like this at some point. Has anyone experienced the same thing as me? I tried using Matrigel again by aliquot it....

12 June 2024 7,269 5 View

Pancreatic cancer organoids not growing well?

Hello everyone, I am currently working on patient derived organoids from pancreatic cancer (PDAC) patients. I noted that my organoids never grow too much. after 1 month in culture they are...

04 June 2024 646 7 View

Immunofluorescence staining and imaging of endothelial tubules?

Hello! I am trying to stain primary endothelial cells that have been grown on Matrigel to form tubules. I am interested in apical-basal polarity. The cells do form tubules but it is hard to image...

28 May 2024 5,855 4 View

How to pellet adipocyte spheroids?

I am using the hanging drop method to develop individual differentiated adipocyte spheroids from the stromal vascular fraction of adipose tissue. With our current timeline they end up 100-300um....

27 May 2024 1,303 1 View

IPSC to motor neurons?

Hi I have following questions regarding iPSC-derived motor neurons: 1. Is it possible to dettached mature motor neurons using accutase and reattach them on coated plates (matrigel, geltrx etc)?...

01 May 2024 3,047 0 View