Does glycerol in protein buffer interfere with NMR studies?

Hi, Does 5% glycerol added to protein buffer (PBS, pH 7.2) interfere with following NMR studies? We are planning to perform NMR based line-broadening experiments to determine small molecule's...

01 December 2020 3,559 4 View

Is it safe not to remove contaminated concrete though mechanically sound if sacrificial anodes would be installed at the corner of the patch?

During repair of concrete structure, I understand that it is not necessary to remove mechanically sound concrete though contaminated while repairing a structure if Impressed Current Cathodic...

08 November 2020 6,861 3 View

Is it AI research development for human benefit or harm?

the technology development is growing up further day to day and mostly based on researches So, is this research development will be more beneficial or harmful to human future? if we think little...

29 April 2020 5,260 0 View

Which language is more convenient to do work on deep learning?

I search through the internet it seems to me that Python is better to work on it. Share your opinion with experience if you agree or not.

08 April 2020 6,002 46 View

How do I make nanoparticle of catechin?

I want to make nano particle of catechin. I have powder catechin 91% which is derived from Uncaria gambier . Please suggest me simple laboratory method to make the nano particles.

23 November 2018 1,610 3 View

Must 'theta' be normally distributed in constructing a L-sigma control limits of a control chart? Say UCL = E(theta) + L*SD(theta). Any reference(s)?

Probability limits can be used if theta follows say a gamma or beta distribution, but I am not sure of using probability limit approach for some charts like EWMA or CUSUM. I would like to know if...

31 January 2017 5,182 4 View

If im using reverse phase HPLC using C-18 column, did i need to used RP-C18 TLC plates when performed thin layer chromatography too?

11 October 2016 6,143 4 View

Can anyone share with me how to identify brain/cerebral tumorigenesis in mice?

I want to know if there is a symptom that can suggest cerebral/brain tumorigenesis has occurred in mice. Maybe behavioral changes or an article that says when cerebral tumorigenesis occur in mice

18 March 2015 2,453 3 View

What is the Hammer Account in LS-DYNA software?

After making a model in LS-DYNA and save it i saw in some pdf tutorial they said it to copy the save file into hammer account. So what is this hammer account?

15 December 2014 9,709 1 View

Phychostimulant use and the brain?

I am interested in substance abuse research. I have a novel idea for a phychostimulant plant used in Africa which I want to explore but I am confused on how to begin. I need help on 1. The best...

25 January 2014 8,403 10 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View

Which alginate is best for making gel for wound dressing application?

We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...

15 February 2021 4,319 4 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View