31 March 2024 2 2K Report

I am trying to extract DNA from serum. I found an article that claimed simple extraction can be done in a single tube - Article Simple procedure of DNA isolation from human serum

Here they used a solution containing 6M NaI/13mM EDTA/0.5% sodium N-lauroylsarcosine/10 µg glycogen as a carrier/26mM Tris-HCl, pH 8.

But currently, I don't have NaI and glycogen. So, I am thinking of making a solution with KI + EDTA + sodium lauroyl sulfate + Tris-HCl, pH 8. And finally, use Na-acetate and absolute ethanol for the precipitation of DNA.

What consideration should I take into account to use alternative reagents?

And, in their protocol does it mean the final solution containing all reagent should have a pH 8 or it just means the use of Tris-HCL with pH 8?

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