11 February 2024 1 7K Report

After a failed CRISPR cas9 transfection in CHO cells, I think the reason was the usage of sgRNA that is matched for Homo Sapiens ACTB gene. Currently I should use this primer, so the other option is to change the cells. Are there any cell lines similar to CHO but of human origin with the following features?

  • Easily growing, attached cell line.
  • Easily grown on regular DMEM media.
  • the cell must not have any endogenous current.

    • Thank you in advanced

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