i want store ATP powder more stable to use for inflammasome activation. For now, i stored it in -20, powder form. Is there any procedure to prevent atp from changing it to ADP for long term
Neutral pH ATP solutions stored frozen -20 are stable for at least a year
For examle ATP solution with RPMI 1640 medium with control of pH via buffers, do you have any suggestion to minimize effects of buffers to ATP?
sorry, it is not clear what you mean. In the complete liquid rpmi medium (+FCS, etc) in the fridge ATP will not stay for long.
ATP in solution has a very low pH. It should be neutralized to 7.3-7.5 before addition to any medium, or storage at -20 to -80C.
normally i used it as powder and fresh for each experiment. And i stored it in -20 already. I just want to find out if it is stable in -20 as powder or not. İ want to be sure that ATP could stay in -20 w/o any dissolution.
Factors that stabilize ATP, pH neutrality near 7, high concentration of solution, low temperature (below freezing point), complete absence of Mg2+ ions or other divalent cation that stabilize the hydrolysis product..
I make ~100 mM ATP pH 7 in water (actual concentration via A260, e=15.3 mM^ -1), and store at -20 degC in aliquots large enough for one day's worth of ATPase activity assays. I usually run out of aliquots in ~6 months.
The original bottle is kept at -20 degC in a sealed jar with dessicant.
Sigma A-7699 says the dry powder is stable for 2+ years. Aqueous solutions for months (-20 degC), or one week (0 degC).
Store ATP at -20º or -80ºC did not modify its stability. But it is recommended to freeze the solution very quickly (as with liquid nitrogen) to avoid the transition temperature and then maintain at -20º or -80ºC.
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