I would like to send Avi.p isolates for serotyping by the kume and Page scheme.
Is this service available and if so, which lab perform it?
For WGS, we need to obtain pellets of Avibacterium paragallinarum (Pasteurellacea-like baceria). What are the preffered centrifuge conditions for this bacterial family? Thanks
01 May 2024 8,030 4 View
We have recently aquired plasmids with the following configuration: EF1A>{gene of interest}:P2A:Bsd When used for transfection (293FT cells and SH-SY5Y cells), these cells expressed the...
11 March 2024 5,049 10 View
The components parallel to the ground of the Coriolis force and the centrifugal force, at mid-latitudes (40°N), are respectively 2ωvsenφ≅4.67×10^(-4) ms^(-2), 1/2ω^ 2Rsen( 2φ) ≅1.62×10^(-2)...
20 January 2024 5,475 4 View
For diagnostics of Avian infectious bronchitis viruses, I use an end-point RT PCR reaction, and before the RT srage I do a primer annealing stage with the target reverse primer (2uM). My queation...
22 August 2023 1,600 1 View
Im trying to find an oligoprimer set for HA gene amplification of H5N1? I will appreciate if you could point out a primer set for phylohenetic analysis by H5N1 HA gene sequencing.
27 November 2022 9,614 2 View
In our diagnostic lab, we extract pure viral RNA (Qiagen viral RNA extraction kit). Therefore, no 28S and 18S rRNA can be traced in agarose gel for total RNA integrity assesment. However, very...
24 October 2022 6,952 7 View
Hi to all, We successfully created in our lab a new RT-PCR system, designed for amplification of the S1 segment of Infectious bronchitis virus (IBV). Yet, we obtained the end point PCR product...
27 October 2021 7,948 2 View
Hello, We have a qRT-PCR system (primers and probe) that does not detect the QX variant. Does anyone knows a universal set of primers and probe for universal detection of IBV? Thanks in...
02 June 2021 7,273 9 View
Hello, Which factors or pathways regulates ETV1 expression? Many thanks !! Ido
13 October 2019 7,502 2 View
the silica nanoprticles may be dissolved to sodium silicate
07 July 2018 1,468 3 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
06 August 2024 8,981 8 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View