Antibody used to stain for expression of inducible surface protein.This is a primary conjugated antibody for flow cytometry. Fluorophore: Brilliant Violet 711 (Similar to Qdot711); Considered to have very bright stain index.
Generally speaking, there should not be any problem when you use a little different concentration of the Ab to stain your cells if you have proper isotype control. To save your money and lower background, you can titrate your Ab around the recommended concentration whenever you receive it, choose the one produce best staining index.
Generally speaking, there should not be any problem when you use a little different concentration of the Ab to stain your cells if you have proper isotype control. To save your money and lower background, you can titrate your Ab around the recommended concentration whenever you receive it, choose the one produce best staining index.
Duraton of incubation and temperature could influence the antibody concentration. Make a dilution raw and incubate your cells over night at room temperature and look which dilution is the best.
The concentrations recommended by manufacturers are for guidance only. In fact, most of the specification sheets received with antibodies contain a statement "The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user", so something similar. Often if you search the literature for the use of a specific antibody putting a catalogue number and manufacturer as key words you could find that antibody concentrations quoted for the same method vary, as the researchers adjusted the concentration to have the best results. Both Shigu and Ute gave you a good advice - titrate your antibody, test several dilutions and chose the one which gives you the best staining index.
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