How can I know which family/species of virus is making the difference between my samples?

I have several metagenomic samples, from which I got the viromes through a bioinformatics pipeline. I made a PCoA of those samples, and some of the samples cluster together. I would like to know...

08 July 2020 8,434 2 View

If female mice are put together on the same cage, are all of them on the same estrous cycle stage?

I work with female mice and I have this doubt. If they are all put together on the same cage, are all of them on the same estrous cycle stage? If so, is there any literature that supports...

01 April 2020 6,266 10 View

How to blast a paired end fastq file?

Hello everyone, I have a paired end fastq file and I know that BLAST+ in command line, accepts fasta format. But I don't know how does it work for a paired end fastq file (I mean in two different...

22 May 2019 2,482 2 View

260/230 ratio of my RNA samples are very low. Is there any method to try purify the sample and synthesize cDNA!?

I extracted RNA from spinal cord tissue using Trizol and following invitrogen's protocol for RNA extraction for PCR. I had good 260/280 ratios (around 1,8-1,9), but very low 260/230 ratios...

16 April 2019 768 2 View

How to perform KASP genotyping of zebrafish larvae at 72hpf?

Dear all, I need to genotype some zebrafish larvae at 3-4dpf. I currently tried to perform it as I do it for the adults, but it did not work. Basically: 1. Fin clip and add the fin to a 96 well...

20 January 2019 989 3 View

IHC in peripheral blood smear?

Hi everyone. We want to do an IHC in peripheral blood smear but it's our first time, and we have some doubts. How do we have to fix the samples? Which is the best fixation agent, acetone, methanol...

15 October 2018 9,338 5 View

Non-transcriptomic pathways that can increase lipid accumulation?

I am currently doing a project investigating the lipid accumulation potential of a chemical using an in vitro model. Upon observing significant lipid droplet accumulation at various...

18 March 2018 4,812 4 View

Are you studying affects toward science, technology and/or mathematics in university and preuniversity students in your country?

We may share instrument and compare data with ours.

02 January 2018 1,064 12 View

QPCR stopped working for well-set up amplicons

Hi all, I ask for your help to solve a difficult problem I'm experiencing with some samples. I'm performing ChIP-qPCR of a transcription factor. We have done this experiment many times and it has...

29 June 2017 6,286 3 View

Is there a protocol to show cytoskeleton disruption in cells after being treated with cancer drugs?

22 January 2017 8,949 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View