How can I know which family/species of virus is making the difference between my samples?

I have several metagenomic samples, from which I got the viromes through a bioinformatics pipeline. I made a PCoA of those samples, and some of the samples cluster together. I would like to know...

08 July 2020 8,434 2 View

If female mice are put together on the same cage, are all of them on the same estrous cycle stage?

I work with female mice and I have this doubt. If they are all put together on the same cage, are all of them on the same estrous cycle stage? If so, is there any literature that supports...

01 April 2020 6,266 10 View

How to blast a paired end fastq file?

Hello everyone, I have a paired end fastq file and I know that BLAST+ in command line, accepts fasta format. But I don't know how does it work for a paired end fastq file (I mean in two different...

22 May 2019 2,482 2 View

260/230 ratio of my RNA samples are very low. Is there any method to try purify the sample and synthesize cDNA!?

I extracted RNA from spinal cord tissue using Trizol and following invitrogen's protocol for RNA extraction for PCR. I had good 260/280 ratios (around 1,8-1,9), but very low 260/230 ratios...

16 April 2019 768 2 View

How to perform KASP genotyping of zebrafish larvae at 72hpf?

Dear all, I need to genotype some zebrafish larvae at 3-4dpf. I currently tried to perform it as I do it for the adults, but it did not work. Basically: 1. Fin clip and add the fin to a 96 well...

20 January 2019 989 3 View

IHC in peripheral blood smear?

Hi everyone. We want to do an IHC in peripheral blood smear but it's our first time, and we have some doubts. How do we have to fix the samples? Which is the best fixation agent, acetone, methanol...

15 October 2018 9,338 5 View

Non-transcriptomic pathways that can increase lipid accumulation?

I am currently doing a project investigating the lipid accumulation potential of a chemical using an in vitro model. Upon observing significant lipid droplet accumulation at various...

18 March 2018 4,812 4 View

Are you studying affects toward science, technology and/or mathematics in university and preuniversity students in your country?

We may share instrument and compare data with ours.

02 January 2018 1,064 12 View

QPCR stopped working for well-set up amplicons

Hi all, I ask for your help to solve a difficult problem I'm experiencing with some samples. I'm performing ChIP-qPCR of a transcription factor. We have done this experiment many times and it has...

29 June 2017 6,286 3 View

Is there a protocol to show cytoskeleton disruption in cells after being treated with cancer drugs?

22 January 2017 8,949 3 View

How to compute the internal consistency of a scale with ordinal items?

Hi community, I am trying to determine what is the best way to calculate the internal consistency of a newly developed self-esteem scale. I am working together with two other researchers on the...

28 February 2021 1,369 5 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

Latent profile analysis based on two variables/sources of data?

Hello, for my master project, I would like to conduct a latent profile analysis about self-regulated learning skills. I would like to take two different measures into account when creating the...

21 February 2021 2,326 3 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

Any suggestion on self-efficacy scale ?

Dear researchers, I am planning on conducting a research on the relationship between Language learning strategies, by Oxford (1990) and self-efficacy. I have found Morgan-Jinks Student Efficacy...

22 January 2021 8,432 3 View

What are best free Molecular Dynamics software packages to generate pre-built biological bilayer membranes?

Our task is to numerically invetigate cellular bilayer membrane. In order to numerically simulate behavior of biological complex membrane, we need to have initially constructed membrane. However,...

13 January 2021 7,809 4 View