Raquel Campos: What specific data are you looking for?

On the web I found this:

"Table 2. Characteristics of 12 μm Sephasil Peptide media.

Peptide C18

Base matrix: silica

Bead form: spherical

Nominal particle size (um): 12

Mean pore diameter (Å): 100

Spec. pore volume (ml/g) 0.7

Spec. surface (m2 /g) 300

Bonded phase: C18

Carbon content: 16%

pH working range: 2-8 ".

*General precautions should be followed with all HPLC columns. e.g. Do not overload or foul the column, Maintain pH under 8 and above 2.5, operate under 5K psi max, wash with a solution that is "stronger" than the mobile phase after use etc.

Q: Does your column have the 12u particles inside (the label should show the particle size)? If so, this looks like the correct data. When Pharmacia sold columns, they often rebranded other vendors generic columns (re-labeled). It appears to be a very old type of C18 (maybe type A or possibly B) and I would not recommend using it for method development (because you will never be able to reproduce it since the column no longer exists). The pore size is shown as 100A (OK for low Mw samples and/or very small peptides). If you are just using the column as a student to gain experience with HPLC and run some samples then it should be fine for generic use. Columns are very inexpensive consumables used with HPLC systems. *Start with a brand new column for anything of value.

BTW: You show two different column dimensions in your question, 4.6 x 100 and also x 250mm.

Thx William Letter! In fact, we just want to train an undergrad to start using our HPLC system with an old column first, so he gains confidence before purchasing a new one. Since I have limited experience working with C18, the routine working pressure was an initial concern.