31 January 2023 1 351 Report

I'm looking for a protocol for measure SDH activity in skeletal muscle homogenate. Are there any protocols that do not include NaCN or KCN?

More Kristina Sharlo's questions See All
How to validate regridding results of environemental data to higher spatial resolution in R?

I am working with several continous, environmental marine datasets at varying original spatial resolutions. My aim is to regrid each dataset to one common, high resolution grid. I am currently...

22 February 2021 2,290 2 View

How to do a Repeated measures analysis with not normal distributed data?

I have results from a listening test in 12 sound environments which are build up out of 3 room properties and 4 occupancy rates. Since the sound samples were often intelligible, subject scores...

04 November 2020 5,423 11 View

Can I use my DNA after the tube was "sterilized" by UV light?

I left tubes with isolated DNA drying (before resuspension) in a biosafety hood for too long and a colleague came and turned on the UV light for some 20-30min. The light did not shine on the DNA...

29 September 2020 536 3 View

How could I avoid mutations upon transforming my gRNAs into the lentiCRISPRv2 vector?

After I have cloned my gRNAs into the lentiCRISPRv2 vector and sent the construct for sequencing, I have noticed that a single mismatch between my clone and original sequence has been introduced....

11 September 2020 4,066 1 View

DNA extraction of swabs stored in ethanol?

Hi all, I am using buccal swabs of seals as a sample source for microsats and mitochondrial sequencing. There is a few samples that were shipped stored in ethanol. , i.e. swab of the buccal area...

08 September 2020 1,013 1 View

How do I interprete the results of a moderation analysis with a categorical and a continuous variable?

Hi all, I have a question concerning a moderation analysis within an experimental setting. The independent variable is categorical and has two options. The moderator is a continuous variable. I...

06 January 2020 9,511 1 View

How to run a binary logistic regression with two experimental treatments?

Hi all, is it possible to run a binary logistic regression within an experiment? That means that the two experimental treatments (treatment 1 vs. control) predict a categorical variable...

03 December 2019 7,319 9 View

What method should I use to freeze the human kidney tissue samples?

Thehuman kidney tissue samples will be used later for immunohistochemistry, ELISA, gene analysis, etc. Should I freeze it in solution or not?

20 June 2019 3,052 3 View

How do THP-1 cells normally look like after PMA treatment?

We just started to culture THP-1 cells and treat them with PMA (100nM) for 72 h. Afterwards we change the media daily as recommended. Does anyone have experiences with serum-free culture in the...

23 October 2017 4,566 1 View

How do I understand the output of my covariate in the Repeated measures ANOVA design?

Dear Leard team, I am currently experiencing some issues with my Master thesis data analysis and I would appreciate your help!!! First I will explain you my study design and then I will explain...

25 April 2017 4,467 3 View

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I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

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What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

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Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

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22 February 2021 8,023 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

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Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

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