Are there any colorimetric succinate dehydrogenase activity assay without CH?

31 January 2023 1 362 Report

I'm looking for a protocol for measure SDH activity in skeletal muscle homogenate. Are there any protocols that do not include NaCN or KCN?

Gordon L Warren

See the attached Munujos et al. (1993) article for alternatives to using cyanide in the succinate dehydrogenase assay.

Badges
Science method

More Kristina Sharlo's questions See All

Merging profiles after change in name due to marriage?

Hello- I have two profiles in ResearchGate. One under my married name of Kristina Secnik and one under my married name of Kristina S Boye or Kristina Boye. How do I merge my publications so that...

29 July 2024 4,426 1 View

How to measure the link between a School's digital transformation and competitive advantage?

How to measure the link between a School's digital transformation and competitive advantage? What measurement models to use? Secondary schools are under investigation.

24 July 2024 2,526 5 View

How to measure the competitive advantage of schools?

Maybe you have ideas or a questionnaire?

05 June 2024 2,646 4 View

Seeking Endorsements for My Research Paper Submission on arXiv! 📄✨ Anyone who fulfill the criteria below?

Dear ResearchGate community, I am looking for someone with endorsement rights on arXiv in the fields of computer science - computer vision and pattern recognition (cs.CV). I would like to submit...

06 May 2024 5,999 1 View

Is it feasible to perform immunohistochemistry (IHC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) on a single mouse brain?

For instance, using one hemisphere for brain slice staining via IHC and the other for analyzing neurotransmitter levels through LC-MS/MS? Are there any existing references or studies...

26 March 2024 2,409 1 View

Addiction and recovery: Gender persception?

In addiction and recovery, are there differences between men and women?

29 February 2024 5,309 1 View

PCR testing of Mycoplasma from egg yolk samples?

are there any protocols for extracting mycoplasma DNA from yolk and subsequent testing by PCR?

20 December 2023 7,585 5 View

Possible to use Devanathan cell with corrosion on entry side?

Is it possible to use a devanathan cell to measure hydrogen diffusion during corrosion this way : The entry side (also called cathode side), corrosion of the working electrode generates hydrogen...

23 October 2023 7,423 6 View

How you raise the efficiency of transfection with CRISPR-Cas9 system (knock-outs of genes)?

Hello! Can you give me some tips how to do CRISPR-Cas9 more effectively? Maybe you can give me some advice on this topic. This technology is new for me, I tried to conduct the experiments to...

11 October 2023 7,785 1 View

How can I do CRISPR Cas knock-out troubleshooting?

Hello! I'm trying to create cell lines of HepG2 with separate knock-outs of mirna 101, 93, 30. I need to separately lower the level of mature miR 101, 93 and 30 in my cells. For this purpose I use...

10 October 2023 4,069 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

Expansion thermal of muscle ?

What is the thermal expansion coefficient of human muscle?

27 July 2024 7,190 0 View

For anyone that works with Human Aortic Smooth Muscle Cells(HASMCs), what seeding density do you use when plating?

I am struggling to find a seeding density that will allow my cells to not aggregate together. Anything helps!

24 July 2024 3,460 1 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Does anyone have sources where I can find the contraction ratios (% shortening of muscle length during full contraction) of specific muscles?

I founde some textbooks stating that typical skeletal muscles can shorten up to 70% (optimally up to 30%) during contraction. However, I would like to look at muscles per region. This might seem...

21 July 2024 399 3 View

Does EMG show muscle spasms?

Electromyography waveforms reflect the electrical activity of a muscle. But does it reveal muscle spasms (transient) or cramps (prolonged spasm)? If so, what does it look like?

20 July 2024 5,951 1 View

Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?

17 July 2024 935 1 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View