Kristina Sharlo

4 Questions 9 Answers 0 Followers

Questions related from Kristina Sharlo

Are there any colorimetric succinate dehydrogenase activity assay without CH?

I'm looking for a protocol for measure SDH activity in skeletal muscle homogenate. Are there any protocols that do not include NaCN or KCN?

31 January 2023 381 1 View

What inhibitors should I use for ChIP?

I'm going to prepare a muscle frozen tissue sample ChIP with pan-acetylated H3 histone antibody. What inhibitors do I need to prevent spontaneous deacetylation of H3 and its degragation? What...

19 January 2022 7,231 2 View

Does Fura-2 survive on muscle cryosections?

I'd like to measure sarcoplasmic calcium on a whole muscle and not on isolated myofibers. Is it possible to perform a protocol for Fura-2 loading on a whole muscle and after that put the muscle...

24 January 2020 6,978 2 View

Qiagen micro RNA primers generate PCR product in negative control, but not in positive control. How could this be?

I used Qiagen miRNEasy micro kit, miScript RT II kit and miScript SYBR greenPCR kit with Qiagen custon primers assays for mir-499 and mir-208. No-matrix PCR showed one-peak melting curve and the...

08 October 2019 3,760 4 View