Kristina Sharlo

2 Questions 5 Answers 0 Followers

Questions related from Kristina Sharlo

What inhibitors should I use for ChIP?

I'm going to prepare a muscle frozen tissue sample ChIP with pan-acetylated H3 histone antibody. What inhibitors do I need to prevent spontaneous deacetylation of H3 and its degragation? What...

19 January 2022 7,189 2 View

Qiagen micro RNA primers generate PCR product in negative control, but not in positive control. How could this be?

I used Qiagen miRNEasy micro kit, miScript RT II kit and miScript SYBR greenPCR kit with Qiagen custon primers assays for mir-499 and mir-208. No-matrix PCR showed one-peak melting curve and the...

08 October 2019 3,721 4 View