I would like to identify the putative site of binding domain in C-terminal tail of LAMP1 with Atg5 protein sequence.
I am interested to learn machine learning and Deep learning ,so in this area please suggestions best one
04 July 2023 140 3 View
I have stable cas9 expressing mammalian cells and predesigned crisprna+tracrna (sgrna) for my GOI. However, I would like to clone sgrna into a GFP/RFP plasmid so that I can sort out these cells...
14 February 2016 1,284 1 View
One of Mycobacteria smegmatis mutant strain I am studying is showing growth defect and elongated cells. We are expecting these elongated cells might be due to failure of cell division. we are...
16 June 2015 8,821 3 View
Formation of 1,2,3 triazoles. Can you please give me a reference or schematic diagram? Thank you
03 March 2015 4,446 4 View
I'm studying functional properties of a transcriptional regulator and trying to locate where it exactly binds on to a DNA region. For that I'm doing DNA foot printing using a 240bp DNA of its...
06 April 2014 5,791 7 View
Can anyone suggest how to get a list (identify/filter) of all human mitochondrial proteins containing WXXL motif in their sequence from human mitochondrial protein databases?
25 March 2014 6,819 3 View
I would like to locate/identify the putative site of binding domain in C-terminal tail of LAMP1 with Atg5 protein sequence.
20 March 2014 9,408 4 View
Can anybody suggest some good ways to draw cell cartoons which we find in research or review articles?
22 September 2013 125 6 View
My target protein is low molecular weight membrane protein (LC3 II). It comes out good in cell systems but not in animal lung tissues (white buffy layer on the top of the protein supernatant,...
19 March 2012 3,035 13 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed
30 July 2024 1,061 5 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View