Sureshbabu Angara

6 Questions 10 Answers 0 Followers

Questions related from Sureshbabu Angara

What are the best vectors to express sgrnas + GFP and excluding Cas9?

I have stable cas9 expressing mammalian cells and predesigned crisprna+tracrna (sgrna) for my GOI. However, I would like to clone sgrna into a GFP/RFP plasmid so that I can sort out these cells...

15 February 2016 1,273 1 View

Can anyone tell me how to identify WXXL motif containing all human proteins from databases?

Can anyone suggest how to get a list (identify/filter) of all human mitochondrial proteins containing WXXL motif in their sequence from human mitochondrial protein databases?

26 March 2014 6,809 3 View

Are there any bioinformatics tools or softwares that can locate/identify the site of binding domain between two protein sequences?

I would like to locate/identify the putative site of binding domain in C-terminal tail of LAMP1 with Atg5 protein sequence.

21 March 2014 9,399 4 View

Are there any bioinformatics tools or softwares that can locate/identify the site of binding domains between two protein sequences?

I would like to identify the putative site of binding domain in C-terminal tail of LAMP1 with Atg5 protein sequence.

21 March 2014 9,824 0 View

A good tool for drawing figures and cell cartoons in a research article?

Can anybody suggest some good ways to draw cell cartoons which we find in research or review articles?

23 September 2013 116 6 View

How to separate lipid contaminants during protein extraction of animal tissues?

My target protein is low molecular weight membrane protein (LC3 II). It comes out good in cell systems but not in animal lung tissues (white buffy layer on the top of the protein supernatant,...

20 March 2012 3,026 13 View