Does anyone have barnase-resistant E. coli?

Promega is selling those Flexi-System Vectors. However, they come with Barnase as a selection marker and hence cannot simply be transformed into your usual library-competent E. coli strain. Since...

31 December 2018 9,684 3 View

Detection of pathway activation at the end of an experiment - which methods exist?

Problem: we want to know - at the END of an experiment - if a signalling pathway was activated DURING the experiment. I had read something about a novel technique that allowed this. I think that...

10 November 2018 9,717 3 View

Shall we start a list of predatory/dubious meetings?

Being invited to be reviewer for a conference or to be speaker is tempting for most young scientists. Not being aware of this businessplan I unfortunately accepted to review abstracts for one of...

02 March 2018 1,694 5 View

How to best ask technical questions in Research Gate - or anywhere?

08 September 2017 8,205 13 View

Can anyone help with an antibody/gene to "quantify" macrophages in tissue lysate?

I have tissue lysates only and would like to check how the population of macrophages changes over time after an inflammatory stimulus. The problem I am facing, is that all the markers commonly...

03 April 2014 584 0 View

Discussion about possible ways to improve the current research system

31 December 2013 1,626 10 View

Nature Cell and Science; are they good or bad for science? (discussion about recent article in the guardian)

Just read this interesting article: http://www.theguardian.com/commentisfree/2013/dec/09/how-journals-nature-science-cell-damage-science . Feel free to read it and post your...

11 December 2013 1,619 7 View

Gene expression/function databases

This isn't really a question. I think it'd be nice to share and collect and discuss different databases to explore gene expression. I found BioGPS (http://biogps.org/#goto=welcome) quite helpful,...

03 April 2013 4,757 2 View

Which mouse CCL2 ELISA is working well?

I am looking for a nice price option & a kit that is proven to work well with serum and conditioned medium from cultured cells (bone marrow derived macrophages).

11 December 2012 8,111 9 View

Any recommendations for assay kits to measure ROS in macrophages using FACS?

09 October 2012 3,061 27 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View