Christoph Metzendorf

11 Questions 87 Answers 0 Followers

Questions related from Christoph Metzendorf

Does anyone have barnase-resistant E. coli?

Promega is selling those Flexi-System Vectors. However, they come with Barnase as a selection marker and hence cannot simply be transformed into your usual library-competent E. coli strain. Since...

01 January 2019 9,790 3 View

Detection of pathway activation at the end of an experiment - which methods exist?

Problem: we want to know - at the END of an experiment - if a signalling pathway was activated DURING the experiment. I had read something about a novel technique that allowed this. I think that...

11 November 2018 9,830 3 View

Shall we start a list of predatory/dubious meetings?

Being invited to be reviewer for a conference or to be speaker is tempting for most young scientists. Not being aware of this businessplan I unfortunately accepted to review abstracts for one of...

03 March 2018 1,775 5 View

Are there any biases in "left-/right-handedness" in different professions?

I keep getting the feeling that left-handed people are over-represented in the life-science field. Of course, it´s just a gut feeling. Therefore, I made a little questionnaire using google form to...

10 October 2017 4,309 3 View

How to best ask technical questions in Research Gate - or anywhere?

This is a rhetorical question, as I´ll give an answer. I get asked questions through research gate messaging from time to time or I see questions on RG where I feel that I have the know how and...

09 September 2017 8,298 13 View

Can anyone help with an antibody/gene to "quantify" macrophages in tissue lysate?

I have tissue lysates only and would like to check how the population of macrophages changes over time after an inflammatory stimulus. The problem I am facing, is that all the markers commonly...

04 April 2014 655 0 View

Discussion about possible ways to improve the current research system

Naveen posted this very interesting question about intense competition, asking whether it´s good or bad for...

01 January 2014 1,708 10 View

Nature Cell and Science; are they good or bad for science? (discussion about recent article in the guardian)

Just read this interesting article: http://www.theguardian.com/commentisfree/2013/dec/09/how-journals-nature-science-cell-damage-science . Feel free to read it and post your...

12 December 2013 1,724 7 View

Gene expression/function databases

This isn't really a question. I think it'd be nice to share and collect and discuss different databases to explore gene expression. I found BioGPS (http://biogps.org/#goto=welcome) quite helpful,...

04 April 2013 4,830 2 View

Which mouse CCL2 ELISA is working well?

I am looking for a nice price option & a kit that is proven to work well with serum and conditioned medium from cultured cells (bone marrow derived macrophages).

12 December 2012 8,182 9 View

Any recommendations for assay kits to measure ROS in macrophages using FACS?

I would like to measure general ROS levels in bone marrow derived macrophages using FACS. There are several assays around and I´d like to know which are easy and reliable. This one seems to fulfil...

10 October 2012 3,155 27 View