Does anyone have barnase-resistant E. coli?

Promega is selling those Flexi-System Vectors. However, they come with Barnase as a selection marker and hence cannot simply be transformed into your usual library-competent E. coli strain. Since...

31 December 2018 9,684 3 View

Detection of pathway activation at the end of an experiment - which methods exist?

Problem: we want to know - at the END of an experiment - if a signalling pathway was activated DURING the experiment. I had read something about a novel technique that allowed this. I think that...

10 November 2018 9,717 3 View

Shall we start a list of predatory/dubious meetings?

Being invited to be reviewer for a conference or to be speaker is tempting for most young scientists. Not being aware of this businessplan I unfortunately accepted to review abstracts for one of...

02 March 2018 1,694 5 View

Are there any biases in "left-/right-handedness" in different professions?

09 October 2017 4,237 3 View

How to best ask technical questions in Research Gate - or anywhere?

08 September 2017 8,205 13 View

Discussion about possible ways to improve the current research system

31 December 2013 1,626 10 View

Nature Cell and Science; are they good or bad for science? (discussion about recent article in the guardian)

Just read this interesting article: http://www.theguardian.com/commentisfree/2013/dec/09/how-journals-nature-science-cell-damage-science . Feel free to read it and post your...

11 December 2013 1,619 7 View

Gene expression/function databases

This isn't really a question. I think it'd be nice to share and collect and discuss different databases to explore gene expression. I found BioGPS (http://biogps.org/#goto=welcome) quite helpful,...

03 April 2013 4,757 2 View

Which mouse CCL2 ELISA is working well?

I am looking for a nice price option & a kit that is proven to work well with serum and conditioned medium from cultured cells (bone marrow derived macrophages).

11 December 2012 8,111 9 View

Any recommendations for assay kits to measure ROS in macrophages using FACS?

09 October 2012 3,061 27 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View