Signal phase shift after analytical calculation?

Suppose we have a signal as x(t) = exp(i(omega*t + phi)), after a simple calculation as y(t) = 1/x(t). We can clearly see that there is a phase shift of Pi=180deg. However, using directly...

20 October 2020 4,923 5 View

Any recommendations on the software to do text mining or sentiment analysis on Chinese reviews?

I am trying to do text mining on Chinese reviews. I have tried out many softwares, like the RapidMiner, Chinese Text Analytics, Python. Most of them seem to require certain level of programming...

12 August 2020 9,109 8 View

HEK-293t cell detach but still alive during killing curve set up?

Hi there, I'm trying to build a killing curve for HEK-293T towards hygromycin B for the following transfection experiment. I plated the cell at 1.3X10^5 cell/well in the 24 well plates the night...

30 June 2020 7,368 1 View

Is my enzyme not working?

Hello everyone, I am currently working on a cloning project with 2 vector pBin mCherry (for localization) and pG5H-GFP (for gene expression). I need to clone 1 ATG gene with the size of 1.3 kb...

09 February 2020 7,931 2 View

We transfected HEK cells for a secreted protein and want to confirm the protein is expressed in right MW. Should we collect supernatant for Western?

We transfected HEK cells for a secreted protein and want to confirm the protein is expressed in right MW. Should we collect supernatant for Western? Not sure we choose the right method. We have...

14 May 2019 4,804 4 View

Why should SVD get two equal singular values for a single mode?

Singular Value Decomposition (SVD) is an important method in the area of signal processing. I try to use SVD to calculate the MHD mode structure from experiment data. However, during the benchmark...

10 April 2019 6,118 4 View

How to derive the Boltzmann equation in plasma physics?

Boltzmann equation in plasma physics is a very fundamental equation in kinetic theory. What's the relation of the one in plasma physics and that in thermodynamics.

03 April 2019 4,925 8 View

What the best soltion has good adhesion to CdS thin film?

I'm trying to find a solution to dissolve particles CZTS- about 15nm.This solution has to have great adhesion to CdS layer. Recently, I have tried toluen and hexanthiol but I still can control...

15 March 2019 5,444 2 View

How to clone a gene with long sequence?

Dear All, I'm now trying to amplify a gene with 3690bp from the genomic DNA. I've design 3 sets of primers. First set is flanking the whole gene. Second set is flanking from 1 to 2613bp. Final...

07 February 2019 1,167 1 View

What bending test and torsion test standard are recommend for a surfboard?

I wish to find a bending and torsion test standard for a surfboard (non-prismatic) that is roughly 2000mm long. The closest I've found for bending test is probably those ones that are designed for...

04 February 2019 3,222 8 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How would you assess protein binding adjusted EC50 for a drug that has a binding rate of over 95%?

I'm in need of a reference or a method to come up with a protocol that allows one to get the protein-adjusted EC50 for an antiviral drug in presence of human serum albumin and acid glycoprotein.

27 January 2021 1,402 1 View

Can i use MM-PBSA to calculate the binding energy of a glycan ligand? ad how?

I have a protein-ligand (glycan) complex and want to do an MM-PBSA analysis using AMBERTOOLS after doing MDS (NAMD). What are the files necessary I should prepare for glycan? Note: I used...

30 December 2020 2,658 2 View

How to get structure and topology for glycosaminoglycan (GAG) of arbitrary length?

Hello all, I want to do glycosaminoglycan (GAG) simulation in Gromacs. Initial structure and CHARMM topology for short chains can be obtained from Charmm GUI glycan modeler. But I have to take...

23 December 2020 6,092 2 View

I have proposal to extract HPA from snails, dos any researcher have protocol of HPA (Helix pomata aglutinin) extraction?

Lectin fro Helix pomatia agglutinin used to detect O-Linked glycosylation

21 October 2020 6,139 3 View

Spike glycoprotein expression will be more in cho or hek 293 cell line ?

Spike glycoprotein will express in which cell line ?

22 September 2020 3,131 4 View

What statistical tests can I perform on glycomics data collected from disease cohort across 3 timepoints?

I have a dataset comprising clinical characteristics and glycomics profiles of disease samples (n=100+) and matched healthy controls. So far, I have been able to do classification modeling to...

20 July 2020 2,153 3 View