I am intersted in T4 DNA ligase orthologs
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25 March 2024 4,678 3 View
Dear smart friends, I have a question about sonification. Can you advise me how I can convert human movement recorded by inertial sensors into sound? Is there any available program/application to...
07 February 2024 5,413 3 View
I have a large dataset containing many dates per participant. I want to creat a new variable containing the number of unique dates per participant. I have been trying to figure this out but it...
06 November 2023 4,788 3 View
Hello, I have performed nucleic acid extraction using the CTAB protocol, and in the final step, I eluted the material with 50 ul of RNase-free ddH2O. A portion of this material was subjected to...
12 October 2023 2,930 3 View
We are performing the hemolysis assay in hydrogels which are more solid-like, we are wondering which size of the material should be used, and in which volume of the erythrocyte solution.
27 September 2023 8,886 0 View
I am trying to detect dopamine/norepinephrine in the mouse brain using fiber photometry, but my current isosbestic point for 465 nm is 405 nm with no control for 560 nm. I found out that for...
24 September 2023 371 0 View
I have recently initiated my ChIP assay and am currently in the process of optimizing the conditions for chromatin shearing using sonication. The extraction is from chickpea leaves for a total of...
21 September 2023 1,891 2 View
I'm wondering about media usage in iPSC culture. Most papers/protocols work with mTESR1, but for me it seems that PluriSTEM needs less media change and no overweekend change. Does anyone use...
03 September 2023 6,868 4 View
areas of research on blockchain in education
22 May 2023 5,210 1 View
I have more than a hundred date variables in SPSS. I want to, for each case, select the earliest date of the 100 date values and the latest date and recode these into new variables. Is there a...
21 March 2023 4,837 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View
