Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Does anyone has a protocol for transfecting AML12 cells?

I am optimizing a CRISPR/CAS9 system in vitro before using it in vivo. I would like to transfect AML12 cells with my pAAV-sgRNA-vector to validate editing efficiency before generating virus.

26 January 2021 8,733 2 View

The impact of Methylisothiazolinone on haemoglobin quantification?

Hello everyone, I am a researcher working on a project aiming to develop an assay for Haemoglobin quantification. In our assay, prior to HGB quantification we dilute the sample in a dilution...

20 November 2020 7,316 2 View

Ansys Workbench Project view shows "Solved" but mechanical shows "Solve Required". How to Fix this without resolving ?

Hello everyone I am running a thermo-mechanical simulation on a Linux Cluster. In my Project view all the component systems seem to have solved. There is a file.rst file and and solver.out...

14 October 2020 2,631 3 View

Standard guitar tuning has two distinguished vectors (0 5 5 5 4 5) and (0 5 10 15 19 24). What is required to show they are tangent-cotangent bundles?

The pitch value set in music and guitar group in tablature are connected by adjunction of tangent-cotangent bundles. Tuning g is the tangent gradient to the flow of pitch on guitar. It determines...

04 April 2020 2,681 15 View

Can you ask for user input on pymol?

I want to write a script that asks the user to enter the PDB file so that the script can be entered (it prompts the user to enter it), the user enters the 4 character code, then the rest of the...

23 February 2020 1,184 1 View

Why cani I not find Constantino Manuel Torresd on Research Gate Search Enginge?

I just read a new paper posted here by Constantino Manuel Torres. However when I put his name in the search engine here, it says there is now one here with that name? I wanted to send him a...

13 July 2019 2,496 3 View

How do I design primers specific to transfected plasmid and not genomic DNA?

I recently introduced a Lenti-Puro Guide vector containing a specific sgRNA sequence into a cell line. I would like to sequence the ddCas9-sgRNA cell lines to confirm that each sgRNA is accurate.

04 February 2019 6,681 3 View

How to make culture media more basic (not too basic) without a pH meter or strips?

Hi, A friend of mine suggested that the reaction that makes Salmonella colonies appear black on XLD or TSI agar might show an increased rate at a slightly lower pH. I was thinking of adding a bit...

24 July 2018 2,916 7 View

Any antibodies against MMP2 and MMP9 that are suitable immunohisto?

Hello All. Can anyone suggest antibodies against MMP2 and MMP9 that work well for immunohisto in the mouse heart? Thanks in advance. Bruce

24 November 2016 7,284 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 932 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View