I am planning a point mutation in adult fibroblasts followed by reprogramming into iPSCs. I realised that the proliferation potential of adult fibroblasts is limited. Because of that, they go into senescence before I mutate them and do further reprogramming. In that case, are murine embryonic fibroblasts better than adult fibroblasts?
Ron Hrstka
Yes, the younger fibroblasts should have a better potential for reprogramming following your CRISPR mutation.
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Lokapriya Dasarathan
Ron Hrstka How is it possible to do both CRISPR and reprogramming in those fibroblasts when they go into senescence after very few passages?
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Ron Hrstka
You might want to re-read both your question and my answer. "...younger fibroblasts should have a better potential..."
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