Lokapriya Dasarathan

4 Questions 2 Answers 0 Followers

Questions related from Lokapriya Dasarathan

Are murine embryonic fibroblasts better than adult fibroblasts for CRISPR mutation and further reprograming?

I am planning a point mutation in adult fibroblasts followed by reprogramming into iPSCs. I realised that the proliferation potential of adult fibroblasts is limited. Because of that, they go...

20 October 2023 6,891 3 View

How to take into account the dilution factor when I use automated cell counter?

Hello people, I am using Bio-Rad TC 20 automated cell counter to count my nuclei. I took 2ul of my nuclei suspension and added 8ul water (1:5 dilution) and further added 10 ul Tryphan blue (1:1...

31 July 2023 2,205 0 View

CUT&TAG not working suddenly?

Hello people, I am struggling with my CUT&TAG. I have attached my CUT&TAG PCR library result where I used the H3K4me3 antibody. I used a minimum of 100k HEK nuclei. What more details do...

13 June 2023 8,757 0 View

Why is there a band in negative control (IgG) for CUT&TAG PCR?

Hello fellow researchers, I did a simple CUT&TAG for H3K27me3 in HEK293T cells. I checked my results using PCR. If you look at this picture that I attached, I first thought I had untagmented...

21 April 2023 6,109 2 View