Actually I am working on immobilisation of enzyme for textile processing. Can anyone suggest me the suitable method of immobilization of cellulase for denim fading?
Hey, i have worked on immobilization of cellulase and also worked on denim biofinishing. May be you can refer my two papers that give the details. Hope it will be useful to you.
Encapsulation of endoglucanase using a biopolymer Gum Arabic for its controlled release in Journal, Bioresource Technology
and
Application of Cellulases From an Alkalothermophilic Thermomonospora sp. in Biopolishing of Denims in Journal, Biotechnolgy bioengineering.
We have some articles for enzyme immobilization on ceramic support. Ceramic support is tougher than organic support. And those that are inorganic ceramic supports can be reused again to the imobilization of the enzymes when they have no more significant enzymatic activity.
In addition, it can be said that there is quite popular types of supports as higher conversion to desired products, for example in relation to the surface area of the supports to immobilize the biocatalyst. However, a prospective study must be considered due to the cost of greatly improved support. For example, Is it better to get products from biocatalysis using a low support value / efficiency of 50% compared to the "super improved supports", considering that the biocatalyst also has a small half life for industrial scale?
There are several example of enzymatic uses for textile applications. For example, a novel azoanthraquinone dye has been obtained through innovative enzymatic process using an immobilized laccase (oxidoreductase enzymatic class). Another pratical application has been studied during the BISCOL european project (for detail see www.biscol.unisi.it).
Article A novel azoanthraquinone dye made through innovative enzymatic process