Suggest suitable method to measure protein content in cotton?

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Adam B Shapiro

Do you mean cotton fiber or cotton seed?

Amit Madhu

cotton fibre

Isn't the fiber made entirely of cellulose?

majority is of cellulose 95%, protein matter present as impurity which is to be removed during chemical processing

I have no relevant experience, but I'll try to make a suggestion. Assuming cellulose is insoluble in 8M guanidine hydrochloride, you could soak the cotton in that, which will dissolve and denature the protein. Then spin down the cotton, and measure the protein concentration in the supernatant using the Bradford assay.

thanks