Hi Amy,

I am not sure what pre-made IDT primers you might be using. However, the ReadyMade primer sequences can be found here:

https://www.idtdna.com/pages/products/custom-dna-rna/readymade-inventoried-oligos/readymade-primers

Let me know if you have any questions, and some specifics about what you are exactly trying to do might be helpful (i.e. which primers).

Good luck!

Shale

Hi Shale,

Thank you for your answer. Sorry not to have been more specific in my question. I meant to say, the primers that IDT offer to target specific genes/transcripts, for which they will not reveal the sequence. I would like to target both FCGR2B and FCGR2A independently, and be confident that I was not getting amplification of both in my assay.

Many thanks,

Amy

It depends. Some opt

Hi Amy,

I think you would need to know the exact sequence of the pre-made primers to be sure. So, it would be advisable to design your own primers.

Hua

Hi Amy,

Okay--sorry for the confusion. As Hua said, I'd either design my own primers, or call IDT's customer service line and ask to speak with someone in R&D.

Good luck!

Shale

Yes, I agree with everyone. I used IDT for most my graduate project and I was satisfied. However I designed my own primer using other programs. I agree with Julio for you to call a representative for further clarity.

Thanks everyone for your answers. Yes, I think designing my own primers that utilize the unique sequences in my gene of interest would be better. This way I can be sure I was not getting amplification of the paralogous genes too.

Best Wishes,

Amy

I would contact them and ask for precisions, they probably won't tell you the sequence but they probably have data about specificity of these pre-designed primers.