Amy L Roberts

2 Questions 12 Answers 0 Followers

Questions related from Amy L Roberts

Are IDT primers reliable for highly specific amplification of a genomic region which shares very high homology with its paraologs?

IDT don't give away much information about the sequence or location of the primers, and I need to be absolutely sure I won't be amplifying paralogous genes as I am trying to identify which paralog...

07 July 2018 9,976 7 View

Is a polyacrylamide gel better than a high resolution agarose gel at visualising faint bands of digested products?

I am using the transgenomics surveyor assay to detect the presence of mismatched DNA after hybridisation. Firstly I amplify a PCR product of 300bp. Then I denature and hybridise this DNA, which,...

04 April 2012 7,706 16 View