I am using the transgenomics surveyor assay to detect the presence of mismatched DNA after hybridisation. Firstly I amplify a PCR product of 300bp. Then I denature and hybridise this DNA, which, if my previous assay has been successful, will result in some degree of mismatch within the sample pool. I then digest the hybridised products with a nuclease. If a mismatch is present I should see partial digestion and DNA bands at 100bp and 200bp, in addition to the original 300bp product. The percentage of my mismatched DNA could be very low and therefore the 100bp and 200bp products could be very faint on the gel. Would a polyacrylamide or HR agarose be the best way to visualise this and ascertain whether my assay is working?

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