Are FspI and BbsI restriction enzymes effective at cutting at their specific sites? May you please state your experience if you used them before?

More Raghda A. Elsabbagh's questions See All

Where can I download Bergey's Manual of Determinative Bacteriology?

27 June 2024 8,055 2 View

Why il-37 anti-inflammatory cytokine level increase in serum and arise in tissue rheumatoid arthritis?

inflammatory response can attacked immune system and effect on healthy cells in your body by mistake, causing inflammation (painful swelling) in the affected parts of the body. as well as joints....

10 December 2023 7,661 3 View

For Extaction of RNA from HUMAN , Which sample should be prefered (which will give best results) ? Plasma or Serum ?

when extraction of RNA save the blood with trizol or serum or plasma

07 December 2023 6,637 2 View

What is my self judgment of my shunts outcome after 16 years?

This question has come to my mind as my paper was "self observation after 8 years". My answer to this question is: a very good outcome inspite of difficulties I have faced. I am basing my answer...

24 October 2023 8,745 0 View

Based on a previous question: Do you think that LP valves progress is equal to VP valves?

My question referred to above (3rd and 4th ventricles symptoms in IH) aimed to help for a better understanding of IH problems. This understanding can lead to better diagnosis, monitoring and...

16 September 2023 2,129 0 View

What are the symptoms of increased intracranial pressure in the fourth and third ventricles?

In an MRI, by size lateral ventricles and areas around might have taken more attention. My question above gives a notice to all smaller spaces CSF passes around. This way of consideration of CSF...

12 August 2023 2,868 6 View

What is the protocol if a shunted patient needed antiplatete treatment?

I think it is a relevant question. Dual antiplatelet followed by single in acute stroke as an example. To add more anticoagulation might be needed in AF.

18 July 2023 641 0 View

Should a medical student going to an oral medical exam. feels as if he is going for a verdict of execution? How to avoid the horrible feeling?

This is the feeling of many medical students at all levels. We have to think of ways of improving medical education and evaluation. A fearfull doctor is not the best person to treat his patients....

17 June 2023 2,822 5 View

How can I calculate Standard Error mathematically ?

What is this mean ( ± 0.06) and How can I calculate it mathematically?

11 March 2023 933 5 View

Fluorescent microscopy protocol and preparing the bacteria sample for test ?

I want to know how to prepare a microbial sample for dyeing and examine it with a fluorescent microscope, as well as prepare dyes DAPI and PI.

12 December 2022 4,856 0 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?

17 July 2024 935 1 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

Isolation of Hydrogen Producing Bacteria?

any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...

13 July 2024 2,255 3 View

How to dilute essential oil from plants to assess the biological activity?

I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...

04 July 2024 8,100 4 View

Hello friends In simulating the drug with the liposome, which side of the liposome membrane is the drug placed on? The top leaf or the other?

Several times by placing a restriction on the upper part of the membrane with the aim of preventing the drug from being added from this side, we tried to keep the drug at the top of the leaf, but...

29 June 2024 9,688 1 View

Can I premix the reagent for restriction digestion and make a mastermix like PCR?

Hi everyone, I am currently trouble shooting my restriction digestion, so far it hasnt given me clear bands when I ran gel. I've been adding all reagents minus template, to make a master mix...

24 June 2024 1,171 3 View

Will this 3C Analysis work or are the fragments too small/close together?

I'm doing a 3C analysis shown in the picture linked and attached below with the Bait fragments labeled and the target fragments that will be tested for interaction with the bait labeled with...

02 June 2024 8,038 1 View

Dominique Liger

Hi there,

I've used BbsI enzyme from NEB for RFLP on PCR products and it has worked perfect. As FspI is also a commercially available enzyme (from NEB for instance) I would say it should work OK provided it is used following the manufacturer's instructions.

Raghda A. Elsabbagh

Thanks a lot Dominique!!