Fluorescent microscopy protocol and preparing the bacteria sample for test ?

Raghda Abdulhussein @Raghda-Abdulhussein

13 December 2022 0 5K Report

I want to know how to prepare a microbial sample for dyeing and examine it with a fluorescent microscope, as well as prepare dyes DAPI and PI.

Badges
Science topic

Similar topics
Correction

More Raghda Abdulhussein's questions See All

Where can I download Bergey's Manual of Determinative Bacteriology?

27 June 2024 8,055 2 View

Can the abnormal sperm(morphologically) fertilize the oocyte ?

Abnormal sperm-morphology

24 February 2024 7,725 6 View

Why il-37 anti-inflammatory cytokine level increase in serum and arise in tissue rheumatoid arthritis?

inflammatory response can attacked immune system and effect on healthy cells in your body by mistake, causing inflammation (painful swelling) in the affected parts of the body. as well as joints....

10 December 2023 7,661 3 View

For Extaction of RNA from HUMAN , Which sample should be prefered (which will give best results) ? Plasma or Serum ?

when extraction of RNA save the blood with trizol or serum or plasma

07 December 2023 6,637 2 View

Von kossa stain ?

when I used von kossa stain on a bone section to detect minerals as part of my research the result appeared as shown in the picture despite the fact that I followed all the steps for the stain...

05 May 2023 4,997 0 View

chitosan nano particles ?

What is the normal range of the partial size and the zeta potential for the chitosan nano particles ?

29 April 2023 8,244 1 View

How can I calculate Standard Error mathematically ?

What is this mean ( ± 0.06) and How can I calculate it mathematically?

11 March 2023 933 5 View

How can I create a sensor of magnetic fields with high sensitivity?

I want to use the hall effect. Is there anything published about this idea?

28 June 2022 3,487 2 View

How to design a protein design for drug delivery?

need program effectively simulate a bilayer system for drug delivery before experimental work

26 September 2021 4,385 3 View

What are the differences between market segmentation and product segmentation? which one is the best for a startup ?

My project is related to these questions and I want to inquire about these two terms to know if can use them in the recommendation system

28 December 2020 6,578 7 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What are the best (less toxic to human) methods of microbial lab fumigation?

We are planning to establish a microbial laboratory in our research center and are actively seeking the best disinfection methods. If anyone has any recommendations, please let us know. Thank you.

04 August 2024 1,973 1 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View