Are both anti CD3 and IL-2 required for Treg maintenance in-vitro?

More Rohan Bir Singh's questions See All

Alternatives for Image J on touch screen platforms?

I plan to use Image J for analyzing images, specifically for computing the stained areas on the images. I feel it is an inefficient and time-consuming exercise to analyze images using ImageJ on...

31 December 2019 1,933 3 View

What is the best test for statistical analysis of treatment groups at baseline and different time points?

What is the best test for statistical analysis of treatment groups at baseline and different time points, specifically when the data is missing for some subjects at follow-ups? - The data doesn't...

31 December 2019 2,554 3 View

Why does a central cloudiness develop on culturing cells from bone marrow in RPMI-1640 complete media over 3-4 days?

I am culturing the cells (isolated from bone marrow) after treatment with RBC lysis buffer with the aim to have a CD11c+ DC population at the end of 7 days. I am using 10ng/ml GM-CSF in RPMI-1640...

03 April 2019 5,554 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

No title

03 March 2021 5,360 2 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Md Murshed Hasan Sarkar

IL-2 is enough for maintenance but if you want to stimulate the cell again then you can use anti-CD3 also.

Julie Joseph

You may find protocols using IL-2 alone, aCD3+IL-2 and aCD3+aCD28+IL-2. The difference would be just maintenance vs. maintenance and expansion. So what to use will depend on what you want to achieve.