Can an algorithm come into control and possession of data without the knowledge of or input from a human programmer?

Keeping in mind the adage on machine learning: data in, data out. I am curious whether there are neural network algorithms that could allow an algorithm to control and possess data on its own...

30 November 2020 860 11 View

Where was Fischerella sp. PCC 9339/Fischerella musciola (UTEX1829) isolated?

Dear colleagues, I am currently comparing some genomic features in different representatives of Fischerella genus isolated from different eco-types. Unfortunately, neither PCC nor UTEX collection...

11 July 2020 4,003 12 View

I'm obtaining some variable data from an intacellellular Ca2+ influx assay using Fura-2AM and require some troubleshooting help, can anyone assist?

-I am using Fura-2AM dye with probenecid to permiabilise TR146 oral epethelial cells. -flourescence is detected on a Flexstation. -Results from current experiments vary significaltly to previously...

29 March 2020 8,088 4 View

Tyramide Signal Amplification (TSA) staining on cell lines?

I am using Tyramide Signal Amplification (TSA) staining on tumor cells (human derived xenograft in mice) to stain tumors for a panel of six markers. I am interested in using this same assay to...

08 March 2020 6,929 3 View

There is a methodology to calculate dynamic level in oil wells?

Hi, somebody knows any methodology to calculate the dynamic liquid level of a oil well, without using downhole sensor or acoustic register. I appreciate your response.

28 February 2020 6,512 3 View

Can a research centre upload content published by its team of researchers?

I am wondering if the administrator in our research centre can upload published content for all researches housed within this group, or must authors upload their work individually?

01 February 2020 7,877 3 View

Is there any scientific evidence that there has been an overarching moral decline among (pre)teen students in the last, say, 10 years?

I hear this type of claim bandied about in certain education circles--namely, by k-12 practitioners themselves--that students' behavior, respectfulness, etc. has all but disappeared. I know that...

15 January 2020 455 2 View

Where can I find the parameters of Miltenyi gentleMACS protocols?

I'm planning to use a Miltenyi brain tumour dissociation kit, but without the gentleMACS machine. Does anyone know where I can find the parameters of the machine's programs/protocols?...

16 December 2019 6,150 1 View

What are the best cost-effective apoptosis assays?

My PhD student is working on phytochemical-induced cell death in cancer cell lines and we need to establish if the cell death is apoptosis or necrosis etc. As our budget is dwindling, does anyone...

18 September 2019 6,389 3 View

Using Guess=CARDS in the Gaussian QC software package?

Gaussian provides the ability to pass in custom molecular orbital coefficients using Guess=CARDS. However, it is not clear exactly where the coefficients should be placed. Are they placed in a...

03 July 2019 7,358 2 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View