Helllo,
if I want to set up a process which wants to manufacture molecular Antibodies and I want to separte them from cell debris, DNA etc. as a first separation step, are there any general criterions with respect to the following downstream processes in order to decide whether the product should be in the upper or lower phase? For instance if I want to do Hydrohobic Interaction Chromatography as a next stept, I guess it would be wise to let them go to the lower kosmotropic salt phase, since HIC starts with a kosmotropic buffer. Do you know any other other similar general rules?
Best, Dennis
ORGANIC PHASE whether upper or lower easy to isolate products that are insoluble or partially soluble compounds. ( solvent are dried and compound can be isolated in pure form)
compounds that are highly soluble are treated with surfactants or ionic liquids
for good separation
Hi!
Do you want to stick to aqueous 2 phase extraction only? Calcium chloride precipitation, followed by affinity chromatography might also work well for extraction of DNA from sample containing antibodies.
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