What is the minimal removable particle size for a depth filtration during monoclonal antibodies downstream clarification / harvest?

More Dennis Lorch's questions See All

How to find documens about the approval of the US Occupation Headquaters in Germany in 1946 for a single German person to found a publishing house?

I am looking for documents relating to the permission of the US Office of Military Government in post-war Germany in 1946 to allow a single person to found a publishing house / media firm for...

14 June 2023 7,754 2 View

Possible to use satillite hyperspectral imaging data for agricultural analysis?

Hello, I am not very familiar with hyperspectral imaging and agriculture, but I am very interested in machine learning models used from data generated by hyperspectral cameras flying over farming...

29 August 2021 1,296 2 View

Projects / databases of hyperspectral imaging in agriculture?

Hello, I am just starting to research about applications of hyperspectral imaging in agriculture. I am looking for university project, start-ups etc. which gather large hyperspectral data over...

18 August 2021 2,281 3 View

What is a common ph-Level for cell broth when harvesting IgG antibodies in a cell bank and at a first centrifugation and filtration step afterwards?

Hello, do you know a common ph Value for the first downstream step in IgG mAb facturing? I would guess around 7 but not sure.

26 September 2019 9,542 0 View

Inclusion Bodies up/downstream process - For what do you use EDTA, Triton X, DNAse and Lysozyme?

Hello, am I right that in Inclusion body processes the chemicals mentioned above are mainly used for cell disruption and removal of cell debris? Which of them do I use for solubilization of the...

25 September 2019 8,476 4 View

Aqueous two phase separation (ATPS) - Which are the relevant criterions to decide whether the product should be in the upper or lower phase?

Helllo, if I want to set up a process which wants to manufacture molecular Antibodies and I want to separte them from cell debris, DNA etc. as a first separation step, are there any general...

20 September 2019 9,195 3 View

What are the biggest structural differences between human and mice antibodies with respect to domains, fab, fc, variable constant chain, hinge region?

Hello, what would be characteristical structural/chemical/modificational differences between human and mice antibodies for all or specifif Ig classes? Best, Dennis

12 September 2019 1,907 3 View

What are the biggest structural differences between human and mice antibodies with respect to domains, fab, fc, variable constant chain, hinge region?

Hello, what would be characteristical structural/chemical/modificational differences between human and mice antibodies for all or specifif Ig classes? Best, Dennis

08 September 2019 8,970 3 View

Aqueous two phase separation (ATPS) - Which are the relevant criterions to decide whether the product should be in the upper or lower phase?

Helllo, if I want to set up a process which wants to manufacture molecular Antibodies and I want to separte them from cell debris, DNA etc. as a first separation step, are there any general...

08 September 2019 9,302 3 View

Principal Component Analysis or something alike for nonlinear Parameter estimation?

Hello, the matlab code of estimated parameters of an non-linear differential equation model is options=optimset('MaxFunEvals', 10000, 'MaxIter', 10000, 'TolFun', 0.0001,...

07 February 2018 8,255 4 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How can I improve the quality of lamb milk replacer for machine feeding?

Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...

06 August 2024 6,227 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

How to calculate effect size of AMCE (Average Marginal Component Effect) in Randomized Conjoint Experiment?

I am following Hainmueller, Hopkins, and Yamamoto's (2014) paper for my randomized conjoint experimental data analysis. The link to the paper is provided below. I received a comment from the...

02 August 2024 4,406 0 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

What's biological importance of bacteria &role of microbes improve crop productivity &soil health &role of bacteria as decomposers incycling nutrient?

What are the biological importance of bacteria and the role of microbes to improve crop productivity and soil health and role of bacteria as decomposers in cycling nutrients?

31 July 2024 3,479 5 View

Valeria Tananska

Hi,

Info here:

https://mycourses.aalto.fi/pluginfile.php/398454/mod_folder/content/0/Filtration.pdf?forcedownload=1

Yet,

https://ethz.ch/content/dam/ethz/special-interest/mavt/process-engineering/particle-technology-laboratory-dam/documents/lectures/practica/Ullmann_Filtration_Fundamentals.pdf

https://bioprocessintl.com/downstream-processing/filtration/case-study-comparing-depth-filter-turbidity-recoveries-examining-single-use-harvest-clarification/

Dennis Lorch

Thank you very much for the links.