Aqueous two phase separation (ATPS) - Which are the relevant criterions to decide whether the product should be in the upper or lower phase?

Helllo, if I want to set up a process which wants to manufacture molecular Antibodies and I want to separte them from cell debris, DNA etc. as a first separation step, are there any general...

20 September 2019 9,051 3 View

What are the biggest structural differences between human and mice antibodies with respect to domains, fab, fc, variable constant chain, hinge region?

Hello, what would be characteristical structural/chemical/modificational differences between human and mice antibodies for all or specifif Ig classes? Best, Dennis

12 September 2019 1,769 3 View

What are the biggest structural differences between human and mice antibodies with respect to domains, fab, fc, variable constant chain, hinge region?

Hello, what would be characteristical structural/chemical/modificational differences between human and mice antibodies for all or specifif Ig classes? Best, Dennis

08 September 2019 8,818 3 View

Aqueous two phase separation (ATPS) - Which are the relevant criterions to decide whether the product should be in the upper or lower phase?

Helllo, if I want to set up a process which wants to manufacture molecular Antibodies and I want to separte them from cell debris, DNA etc. as a first separation step, are there any general...

08 September 2019 9,186 3 View

Does anyone has fouling factors heat exchange design for lignin solutions or the aqueous/oil phase after hydrothermal liquefactions or other treatmen.

for u-bundle heat exchanger oder any other heat exchange device

21 May 2017 3,419 3 View

Does anyone have heat transfer coefficients between lignin solutions and the aqueous/oil phase after hydrothermal liquefaction or other hyd. treatment

with a U bundled heat exchanger or any other heat exchange device

21 May 2017 4,176 1 View

Has anyone measured the viscosity of Lignin / Water and catalysts such as Kaliumhydroxide solutions?

15 May 2017 1,357 1 View

How would you calculate the heat capacity of a 1% wt KOH and 13% Ligin solution? I have the cps of dry KOH and Ligin, can I sum up by relative weight?

The Temperature of KOH and Ligin should be room Temperatur around 298,15 K, Water has a fraction with room temperature and a fraction around 353,15 K, but perhaps its ok to consider all components...

06 May 2017 6,661 3 View

Does anyone have heat transfer coefficients between lignin solutions and the aqueous/oil phase after hydrothermal liquefaction or other hyd. treatment

with a U bundled heat exchanger or any other heat exchange device

04 May 2017 6,940 1 View

How would you calculate the heat capacity of a 1% wt KOH and 13% Ligin solution? I have the cps of dry KOH and Ligin, can I sum up by relative weight?

The Temperature of KOH and Ligin should be room Temperatur around 298,15 K, Water has a fraction with room temperature and a fraction around 353,15 K, but perhaps its ok to consider all components...

04 May 2017 6,128 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View