Aqueous two phase separation (ATPS) - Which are the relevant criterions to decide whether the product should be in the upper or lower phase?

Helllo, if I want to set up a process which wants to manufacture molecular Antibodies and I want to separte them from cell debris, DNA etc. as a first separation step, are there any general...

20 September 2019 9,051 3 View

What are the biggest structural differences between human and mice antibodies with respect to domains, fab, fc, variable constant chain, hinge region?

Hello, what would be characteristical structural/chemical/modificational differences between human and mice antibodies for all or specifif Ig classes? Best, Dennis

12 September 2019 1,769 3 View

What are the biggest structural differences between human and mice antibodies with respect to domains, fab, fc, variable constant chain, hinge region?

Hello, what would be characteristical structural/chemical/modificational differences between human and mice antibodies for all or specifif Ig classes? Best, Dennis

08 September 2019 8,818 3 View

Aqueous two phase separation (ATPS) - Which are the relevant criterions to decide whether the product should be in the upper or lower phase?

Helllo, if I want to set up a process which wants to manufacture molecular Antibodies and I want to separte them from cell debris, DNA etc. as a first separation step, are there any general...

08 September 2019 9,186 3 View

Does anyone has fouling factors heat exchange design for lignin solutions or the aqueous/oil phase after hydrothermal liquefactions or other treatmen.

for u-bundle heat exchanger oder any other heat exchange device

21 May 2017 3,419 3 View

Does anyone have heat transfer coefficients between lignin solutions and the aqueous/oil phase after hydrothermal liquefaction or other hyd. treatment

with a U bundled heat exchanger or any other heat exchange device

21 May 2017 4,176 1 View

Has anyone measured the viscosity of Lignin / Water and catalysts such as Kaliumhydroxide solutions?

15 May 2017 1,357 1 View

How would you calculate the heat capacity of a 1% wt KOH and 13% Ligin solution? I have the cps of dry KOH and Ligin, can I sum up by relative weight?

The Temperature of KOH and Ligin should be room Temperatur around 298,15 K, Water has a fraction with room temperature and a fraction around 353,15 K, but perhaps its ok to consider all components...

06 May 2017 6,661 3 View

Does anyone have heat transfer coefficients between lignin solutions and the aqueous/oil phase after hydrothermal liquefaction or other hyd. treatment

with a U bundled heat exchanger or any other heat exchange device

04 May 2017 6,940 1 View

How would you calculate the heat capacity of a 1% wt KOH and 13% Ligin solution? I have the cps of dry KOH and Ligin, can I sum up by relative weight?

The Temperature of KOH and Ligin should be room Temperatur around 298,15 K, Water has a fraction with room temperature and a fraction around 353,15 K, but perhaps its ok to consider all components...

04 May 2017 6,128 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View