Approximate deconvolution (ADM)?

More Sara Jafari's questions See All

How to solve the problem of unconscious teeth grinding during sleep at night?

One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...

08 August 2024 1,366 5 View

Is Galaxy.org good to use for research for analyzing data and for publication?

Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)

06 August 2024 6,610 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to send my account link and how to make my account public?

Nothing

01 August 2024 755 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Should Coefficient of Variation (CV) in a biological data necessarily be below 30%?

In a research, lesion diameter due to a disease has a CV below 10%. However, in an another parameter, disease inhibition percentage (compared to control), a value derived from lesion diameter...

27 July 2024 201 0 View

Non-parametric version of the wo-way repeated measures ANOVA?

Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...

24 July 2024 3,284 6 View

Could someone please provide a list of journals that accept the application of methodologies in nature-based solutions?

I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.

23 July 2024 6,444 2 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Is possible to edit a gene present in a plasmid by Crispr Cas9?

Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...

22 July 2024 7,073 2 View

How can use ImageJ program to separate RBC, WBC and Platelet/Fibrin from thrombus HE staining?

Hello, I need help from you guys. I have ischemia stroke thrombus HE image and I should use ImageJ (color deconvolution) to separate RBC, WBC and Platelet/Fibrin components in it as percentages...

30 November 2023 5,253 0 View

Are there deconvolutional algorithms available that can delineate EMT states from bulk RNA-seq data?

EMT has been well established to be a collective process of distinct intermediate states. I would like to retrieve information from my bulk RNA-seq dataset beyond the generic EMT signature. One...

02 September 2023 3,200 0 View

Can someone please guide me in deconvolution of FTIR data?

Specially I need to deconvolution of Metal bidentate bridging and Metal bidetate chelate peak, Inaddition Linear dimmer and cyclic dimmer

28 July 2023 7,919 1 View

A suitable software for deconvolution of NMR spectroscopy ?

Please suggest a suitable software for the deconvolution of NMR spectroscopy. (Open source). Thanks in anticipation.

25 June 2023 9,546 2 View

As we all know that Si 2p peak can be deconvoluted into two peaks such as Si 2p1/2 and Si 2p3/2. Which peak has higher binding energy and why ? ?

In the few literature, it was mentioned that the binding energy of Si 2p1/2 was higher than Si 2p3/2, while some authors made the just opposite statement about these peaks. I am so confused about...

22 June 2023 1,135 2 View

How to set a proper method of images thresholding using Fiji ImageJ?

Hi everyone, I am using Fiji in order to get IntDen. of some cancer tissues, and I’m following these steps, drag the image Edit, selection, Specify, W=250 H=250. image, color, colour...

29 April 2023 2,639 3 View

What is a step of deconvolution the XPS spectra?

To whom may have a solid background in X-ray photoelectron spectroscopy, The technical deconvoluting way is not an issue for me, I wonder how I can decide the number of shoulder peaks (or hidden...

06 January 2023 7,956 3 View

How to calculate the area of deconvoluted spectra?

13 December 2022 717 2 View

How can I calculate oxygen vacancies in metal oxides by XPS?

Hi everyone, I want to know the ratio of (lattice oxygen : oxygen vacancy : hydroxyl group) of sample which I have. So, I tried to deconvoluting the O(1s) XPS spectrum using 3 Gaussian fitting...

27 October 2022 8,186 0 View

How to get CDNN software for circular dichroism analysis?

Dear RG users, Does anybody know how to get (download/purchase) CDNN, a software for circular dichroism deconvolution data analysis? Best, Adrian

18 September 2022 6,979 19 View