I'm trying to culture hematopoietic stem cells of mice, referring to an article, in which fibronectin-coated plates are used. Since pre-coated plates are expensive, I'm thinking about coating plates by myself.
I found that relatively wide range of amount is on the instruction manuals, such as, concentration of "5-30 ug/ml, 50 ug/ml", amout of "1-5 ug/cm2, 5 ug/m2", or sometimes "depends on the type of the cells". I searched on the internet for the correct answer, but I haven't found one.
Does anyone know the answer?
Daisuke Koga
Sebastian Zanner
Thank you for your answer.
So, you used it with the concentration of 25 ug/ml.
By the way, when the surface area was 85mm2, what plate did you use ?
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