For in silico analysis of a variant, howmany bioinformatic tools are required to give a sign in the favour of its pathogenecity?

Dear fellows, I am wondering about, how many bioinformatic tools are required to declare a variant as pathogenic and deleterious?

02 October 2020 6,580 3 View

Plasmid visualization on gel electrophoresis?

I extract the plasmid from e. Coli, on nano drop its concentration was 60.but no band was formed in gel electrophoresis?I use 1% agrose gel with 2.5 ethidum bromid.

06 September 2020 4,438 11 View

No title

02 June 2019 8,134 2 View

Given in 3D Ellipsoid semi major axis a=5 and b =4 ,c=3 . How can we find the distance D between two focal points (P0 and P2)?

Given in 3D Ellipsoid semi major axis a=5 (along axis) and semi minor axis b =4 ,c=3 (along y and z axis) . How can we find the distance D between two focal points (P0 and P2) ? Also how to find...

09 May 2019 8,093 4 View

How to fix the range of 't' (time) in matlab such that the delta function returns the impulse response for each dealy in matlab?

How to fix the range of 't' (time) in matlab such that the delta function returns the impulse response for each dealy in matlab. Can anyone help me in this regard ? I am having problem setting...

04 May 2019 8,787 3 View

Does surface doping always develop hetrojunction at the interface?

Is surface doping like attachment of noble or other metals at the exterior of nanomaterials? Hetrojunctions are developed in the second case, but dopant is in very small concentration ,would it...

11 May 2018 868 4 View

How the bacteria can be differentiated visually in a mixed culture by adding aniline blue to MRS medium?

I am using mixed culture of LAB and Yeast for cereal fermentation.For enumeration of each bacterium in a mixed culture, I need to add anilin blue to the MRS medium but I didn't found how the...

23 July 2017 9,525 23 View

How to prepare sample for Photoluminescence , if it is insoluble in water?

PL technique requires liquid sample. Can it be used as a suspension for water insoluble materials?

01 May 2017 5,646 3 View

Why we use DNAses in RNA isolation as in end for PCR analysis we have to finally convert RNA to DNA.

08 February 2017 9,218 3 View

What is the best method to analyze mouse bone marrow stromal cell culture?

I am having problem of mouse bone marrow stromal cells primary culture. First 2-3 days I see cells very healthy but then slowly hazzy and dying. I use alpha MEM media with 10% FBS and 1x glutamine...

12 August 2014 7,396 7 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

No title

02 March 2021 3,060 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View