Application of UMAP in flow cytometry?

More Saeede Soleimanian's questions See All

Please guide me to induce MAA synthesis in microalgae about how many hours and at what interval with what dose of UVA and UVB radiation is required?

I want the dose used in my experiment to be less than the minimum lethal effect of these rays And I need a minimum amount for induction for all strains.

31 December 2023 6,428 0 View

What intervals and intensities of UVA and UVB are usually tried to induce the production of mycosporins in different strain of microalgae?

The light distance and the count of cells to be tested are important to me

03 December 2023 6,943 0 View

I would like a valid reference on rapid extraction of mycosporins from microalgae by spectrophotometric method?

A reference that contains the amount of microalgae biomass and the amount of solvents

03 December 2023 9,995 1 View

Do UVA and UVB rays pass through glass and plastic Petri dishes?

In fact, I want to understand the amount of penetration or absorption of these two rays in the amount of algal biomass?Does UVA and B rays pass through my cultivation flask?

13 November 2023 338 1 View

What 40% acetic acid soluble drug do you know that is suitable for wound healing?

I want to make a scaffold for wound healing, the solvent used is acetic acid and I want a medicine that dissolves in this solvent and is suitable for this usage. scaffold base is gelatin and...

26 May 2022 2,120 0 View

How to create realistic carbon nanotube junctions?

Dear all, I want to connect carbon nanotubes as they connect in reality. It means that in the connection area there are pentagons and heptagons. I use CONTUb (an algorithm for connecting two...

04 January 2022 3,834 3 View

Surface roughness in tubes?

Dear all, I want to know about defining surface roughness in tubes. I connect two tubes with different radius, for example have one smaller tube between two parts of larger tube and then increase...

01 March 2019 466 1 View

Does somebody give me an easy and standard protocol for activating C. perferingens?

I am working on the application of probiotic and prebiotic instead of nitrite for preventing Clostridium perferingenes growth in the red meat sausage. I have received C. perferingenes from Iran...

28 April 2017 7,899 3 View

I highly need your help about CMV?

May I ask you some question? I highly need your help I want to infect human foreskin fibroblast with CMV standard strain(AD169) to study NK cell activation with that ,unfortunately, we can 't...

01 November 2016 8,490 2 View

How we can calculate beta sitosterol content of a sample by GC (after saponification and dervatization)?

In order to measure phytosterol concentration of O/W emulsion, I have used single point internal standard method i.e. calculating the response factor, using areas of known amount of IS and the...

28 October 2016 152 5 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View

Flow through curved domains?

Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...

25 July 2024 9,058 4 View

BD Cell Viability Kit - TO evaporation?

Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...

22 July 2024 2,257 0 View

Can I use CCCP instead of H₂O₂ as a positive control for ROS measurement through flow cytometry?

I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.

20 July 2024 6,354 0 View

I came across oscillations in a pressure profile for a pipeline flow along the axis of a cylinder, how do I prove that these are not numerical err?

In terms of CFD, we often analyze the stability of the error using Von-Neumann analysis, especially for FDM based problems. Should we follow the same approach for a compressible fluid flow using FVM ?

13 July 2024 6,296 5 View

Alexandr Chernov

Dear Saeede!

Please You look at the following publications:

https://clambeylab.squarespace.com/blog/2019/5/23/the-umap-plugin-in-flowjo-a-users-review

https://www.appliedcytometry.com/news/umap-high-colour-flow-cytometry/

https://www.appliedcytometry.com/news/using-umap-to-process-high-colour-flow-cytometry-data-part-2/

Saeede Soleimanian

Hi Alexandr

Thanks to answer me.