We would like to evaluate small molecule compound(s), where exact mechanism-of-action is not clear at the moment. We are conducting in vitro studies right now and would like to extend these studies in appropriate rodent model(s). Primarily, our aim is to explore:
1. Full/partial blockage of pre-adipocyte to adipocyte conversion?
2. De-differentiation of fully differentiated adipocytes?
I would say also db/db and not just ob/ob
This may be helpful as well:
http://jaxmice.jax.org/literature/models/diabetes.obesity.pdf
Also, probably just a wild type mouse fed with Western Diet could be useful for your purposes. They still develop obesity (or at least significant increase in body weight) within 4-5 weeks.
Kulvinder,
A possible animal model for your POC efficacy study could be the diet induced obesity model using Nnt-mutant C57BL/6J (B6/J) (Jackson Laboratory number #664). This animal model exhibits significant weight gain by 20 wks when fed a high fat (60% of calories). The reference for the article is:
Diet induced obesity in two C57BL/6 substrains with intact or mutant nicotinamide nucleotide transhydrogenase (Nnt) gene. Obesity 2010 18(10):1902-1905 the article comes from Edward Leiter's lab at Jackson. Another rodent model might be the Zucker fatty rat (single gene mutation). Hope this is of help to you.
Best,
Jorge L. Jacot, Ph.D.
Dear Kulvinder,
I do agree with Dr Salvetor and Dr Jorge.
You can try on Zucker fa/fa rat model also.
Regards
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