Anyone with experience in mitochondrial calcium imaging in neurons?

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Peter H. Roos

Perhaps this reference is of interest:

◾Hausherr, V.; Schobel, N.; Liebing, J.; van Thriel, C.: Assessment of neurotoxic effects of tri-cresyl phosphates (TCPs) and cresyl saligenin phosphate (CBDP) using a combination of in vitro techniques NeuroToxicology 59: 210-221 (2017)

Manoj kumar Jaiswal

You might be interested to see these two references.

Cu/Zn superoxide dismutase typical for familial amyotrophic lateral sclerosis increases the vulnerability of mitochondria and perturbs Ca2+ homeostasis in SOD1G9...

MK Jaiswal, BU Keller. Molecular pharmacology 75 (3), 478-489

Impairment of mitochondrial calcium handling in a mtSOD1 cell culture model of motoneuron disease

MK Jaiswal, WD Zech, M Goos, C Leutbecher, A Ferri, A Zippelius, ...

BMC neuroscience 10 (1), 64.

Manoj

Raghavendra Singh

You can try to depolarize neurons with 60 mM KCl or with Glutamate/glycine. If you are not studying store operated Ca2+ entry by pre-depleting with Tg, then 1uM calcium and 100 uM ATP might not work.