Nrf2-antibody from Santa Cruz (sc-722) gives fluctuating heavy bands
Can we collect scions from 1-year-old grafted plants instead of collecting scions from 40-year-old trees (candidate mother plant)? Are both plants physiologically and genetically the same? If yes,...
17 December 2023 2,790 3 View
Please reply me details about the single crystal Rietveld refinement.
23 November 2023 8,365 1 View
@Arindam Roy clean cooking https://www.aicte-india.org/downloads/Startup%20Policy.pdf
14 August 2023 6,233 1 View
I want to collaborate for future publication in the area of Brand evangelism. BE is a new concept and much research was not done in INDIA and other developing countries. SO anyone interested in...
18 March 2021 2,654 3 View
how do I implement CNN on the dataset rather on the image.
11 September 2020 1,182 5 View
Hello to all respected professors and researchers. Currently, I am going through this topic. After following references, I wrote the Abstract and Introduction. I want to know what other concepts I...
10 June 2020 8,231 3 View
Hello to all respected professionals. Hope you all are fine. I have some doubts. I am sending below. Can you tell me the names of some useful algorithms used in net load forecasting and line...
10 June 2020 8,292 3 View
I have a HPTLC plate but Rf value was initially not considered. How can I justify my findings? Will be the fluorescent band of the sample which matches with the standard is more than enough?
24 May 2018 6,961 6 View
In Google Scholar i can able to upload the publication details but full paper upload details are not available
06 February 2018 1,258 48 View
Hello scientists. Can you please suggest a good T cell line for toxicity assessment? Thanks in advance.
25 April 2016 7,870 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View