I am trying to detect tau in lysates of SH-SY5Y neuroblastoma cells using this antibody. However, I am having trouble detecting any bands. Once or twice I got faint bands, and most of the time I do not get any bands at all. Anyone has had any success using this antibody?

I am loading about 100 ug total protein on the gel. I have tried antibody dilutions ranging from 1:1000 to 1:500. On the antibody vial, they have not given the concentration, only the total amount (100ug) and not mentioned the volume. So I am unable to estimate the concentration of the antibody stock.

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