Working on three different insect genotypes. Need to calculate Effective Time, ET50 for pupation for each insect genotype. Kindly suggest software or methodology to be used.
Yes,first work out LC or LD 50 or 95.Then go with time scale variation with LD or LC single dose administration. Better have very close time slot observations.Rest of the procedure is same. Be sure of ensuring the death of target stage.
Dear Vinod,
As Prof. Shashikant S. Udikeri indicated you should use a single dose and observe the mortality values according to the time intervals. These intervals can be empirically chosen. The calculation is the same as in case of LC-LD values (probit analysis or probit transformation).
Dear Vinod,
For the method, you should do as Prof. Shashikant S. Udikeri and Mr. András Bozsik recommended. i know two softwares to determine LT50, JMP (from SAS institute) and SPSS, and PoloPlus. JMP is a new sofware from SAS. hope it help you
Teguh
Dear All,
For probit transformation you do not need a computer but a probit table and a calculator which can count linear regression. By the way, it can be done even without calculator.
Dear Mr. András Bozsik,
I agree with you. before we use the software, we should be know about probit analysis throughly. for table probit i only know from this book (as attachment, i took it from IRRI, it;s free e-book) and from D.J. Finney. hope it will be use for you Mr. Vinod Kukanur
Dear colleague,
You can use a linear method (probit analysis) or a non linear method (using Prism v 6.01 for Windows (GraphPad Software Inc., www.graphpad.com). The conventional and easy method for you is the the probit analysis. Mortality was assessed using a series of doses and corrected (Abbott, 1925). Bioassay data were subjected to probit analysis (Finney, 1971), and estimated lethal doses (LD50 and LD90 values, that is, the dose causing mortality effects in 50 and 90% of the treated insects, respectively) and their fiducial limits were determined (Swaroop et al., 1966).
Abbott WS (1925). A method for computing the effectiveness of insecticide. J. Econ. Entomol. 18: 265-267.
Finney DJ (1971). Probit A nalysis, Third edition. Cambridge University Press, London. p. 333.
Swaroop S, Gilroy AB, Uemura K (1966). Statistical methods in malaria eradication. Geneva: World Health Organisation. Monograph Series. 51: p. 164.
Good luck Pr. Noureddine SOLTANI
Thank you...András Bozsik Sir, Teguh Rahayu, Noureddine Soltani Sir....for suggestions and providing information. It helped a lot....
If you are really being good about this the important thing is dose acquired over time as a function of body mass. So you need multiple doses and multiple observation times. This is then a time-dose-mortality trial. There are not many examples of this in the literature. However, here is one:
Oral toxicity of essential oils and organic acids fed to honey bees (Apis mellifera)
Article in Journal of Apicultural Research · November 2007
Impact Factor: 1.36 · DOI: 10.3896/IBRA.1.46.4.02
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