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 Freshly prepared sterile YEPD broth medium was inoculated by fresh culture (approx. 2×103 cells) of test organisms in different conical flasks. The cells were exposed to the various concentration of test compounds separately.  All the conical were incubated at 37°C and 160 rpm. At predetermined time points (0, 4, 12, 24, and 48 h after incubation with agitation at 37°C), a 100-µL aliquot was removed from every solution and appropriately diluted in sterile water. A 100-µl aliquot from each dilution was streaked on the YEPD agar plate. Colony counts were determined after incubation at 37°C for 24 h. Time-kill curve was determined by plotting mean colony count data (log10 CFU/mL)  as a function of time for each isolate.

Good answer Mohammad Irfan