What are effective methods for removal of iron from organic acids?

I am seeking methods to remove trace iron from organic acids, including acetic acid, propionic acid, and formic acid. Distillation works, but is expensive. I'm looking for alternatives to...

31 January 2021 984 3 View

When using explanatory sequential mixed methods, why do you need to use this method when the population groups are uneven?

I am currently working on my dissertation. I wanted to do a quantitative study using 3 groups. Two of the groups are approximately the same size, but the third group is significantly smaller....

01 May 2020 6,561 3 View

Qualitative data set from interviews or conversation?

I'm looking for a qualitative data set (preferably one that has already been published) that has transcribed and coded from interviews and/or small group setting. The topic area is not important....

27 December 2018 6,840 6 View

Can I generate species rarefaction curves based on two different trapping techniques?

I am using two types of traps to collect insects and would like to predict the species richness of different localities. Is it possible to generate rarefaction curves for both methods combined or...

14 March 2018 553 3 View

How do I get a free copy of Primer?

Is it possible to get a free copy of Primer. If not is there another statistical software which is good for analyzing diversity indices. Thank you.

10 December 2017 6,066 4 View

Adding jpg files of paper?

I want to add information for my Fluorine Electron Microprobe Analysis talk at an 1982 conference. There are 3 jpg images of the paper. [ I'm trying to get a pdf file ] Is that possible?

25 February 2016 3,551 6 View

Does a defendant that has fetal alcohol syndrome have some protection in a misdimeanor court case?

The defendant was 16 years old and was doing graffiti and was also charged with possession of tobacco. The defendant had been diagnosed with fetal alcohol syndrome.

12 March 2015 3,807 10 View

How can I optimize QIAamp Fast DNA Stool Mini Kit for RNA isolation?

Hello, I will be using this kit provided by Qiagen for isolation of viral RNA from stool samples. I was wondering if anyone had any input on how to tweak the protocol to allow for efficient...

15 January 2015 7,114 4 View

Any suggestions for a water-immiscible solvent with reasonable SDS solubility?

27 October 2014 5,077 5 View

What are the benefits of a ResearchGate account versus the costs of the protracted process of opening one?Enter a question

06 July 2014 3,613 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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03 March 2021 5,360 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View