Are there commercially available GFP-Expressing HUVECs for angiogenesis assay which have not been infected with lentivirus?

Dear all, I am interested in setting up an Angiogenesis assay on our Essen IncuCyte system but the cells which are recommended are infected with Lentivirus which we can not use due to our labs...

29 November 2020 2,350 1 View

Interaction terms and singularity errors?

Hi, I am currently trying to produce a linear regression model. I have used hierarchial regression stechniques to produce a multivariable model. I have then looked for interaction terms and added...

03 December 2019 4,201 3 View

Best Protocol for Biosynthetic Gene Cluster Heterologous Expression?

Hi, Does anyone have any recommendations on what methodology to use for the direct cloning and heterologous expression of a 69kb NRP gene cluster from Gram-positive bacteria? Thank you!

06 November 2019 3,179 3 View

Designed primers ran on a gel, not working?

Hi, I am looking for advice. I designed my own primers for human TBP, TGFB1 and ITGB6 Gene's. To test my primers I ran a PCR and gel, however, it didn't work. Does anyone have any solutions? I...

20 March 2019 3,950 3 View

Does anyone know what this rectangle on my western blot is?

I have been running western blots to verify the presence of a protein in my co-IP but a strange rectangle keeps showing up on my membrane and blocks the signal when I image it. I reran my western...

16 January 2019 847 4 View

Caco2 cell line passage number and Pgp presence?

What is the passage number of caco2 cell line that has the highest expression of Pgp? I remember that there was a range at which the expression increases but after that passage number it is no...

11 September 2018 7,436 1 View

How do I clear Mycoplasma arginini contamination from my viral RNA sample for NGS sequencing?

Hello, I have extracted my RNA from a virus and I want to sequence using NGS, the RNA sample has Mycoplasma arginini contamination. I would like to eliminate the bacterial contamination before...

06 March 2018 7,380 2 View

C10 cells not growing?

I just started working with an immortalized C10 cell line. The new batch was defrosted by standard methods but is not growing. Often not dying, but not proliferating. Cells have long projections...

11 September 2017 3,317 3 View

Anyone know how to do a data dictionary for a System Dynamic model and develop a user friendly web based tool for public use of the SD model?

What software to use, previous works done in the same area?

04 May 2016 8,367 5 View

Any advice on measuring phenolic content?

08 October 2015 8,010 10 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View