Hello - I am working on a project when I am doing a LAD ligation in a Rat heart and looking for a stain I can use to mark the spot and distinguish it during sectioning on the cryostat. Anyone have an idea of what to use?
Thank you for the replies, I can explain the situation more.
After LAD ligations, we will be explanting the heart and using a probe to measure some of the heart's characteristics. Following this, the hearts will be briefly frozen (-20 for 10 minutes) and sectioned using a heart slicer matrix, then stained. I would like to mark the spots on heart where I probed so that I can correlate the readings from the probe with images of the stained sections. We will not be doing OCT perfusion / cryosectioning.
I am looking for the best way to do this marking of the heart. I have considered using different inorganic dyes, Coumasie stain, or even a marker, but I was wondering if anyone has experience in doing this type of marking.
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