Samir Zaman

6 Questions 5 Answers 0 Followers

Questions related from Samir Zaman

Can I still measure insulin, free fatty acids, or triglycerides from serum that was left unseparated from RBCs at 4c for >12 hours?

I took blood from mouse tails for serum collection and left them in a temperature controlled centrifuge at 4c overnight. The serum I collected was pink-tinged, but still fairly clear. I would like...

13 October 2021 3,741 4 View

Can isolectin b4 be removal / elution from unfixed tissue sections?

Hello, I am using FITC-conjugated isolectin b4 to look at perfused vasculature in the myocardium. However, i would like to be able to remove the lectin from the tissue section so that I can probe...

21 March 2018 1,007 0 View

Why can I not detect beta-actin on my blots?

**EDIT: 30ug of Protein is loaded measured by microBCA I've been trying to blot for beta-actin on cell culture protein lysates for weeks without success. The image below is the best I have...

10 November 2017 9,090 3 View

Can adherent luciferase-expressing cells luminescence in response to D-luc while in suspension?

I'm working with HEK293 cells stably expressing a lentiviral CMV-FL construct that responds to luciferase when plated.Does anyone know if these same cells would still luminescence while in a...

30 September 2016 849 2 View

Best brand for Rat Heart Slicer Matrix?

I'm looking for a durable matrix slicer to make coronal slicers of an adult rat heart in increments of 1 mm. I'm looking currently at Zivic Instruments and Cell Point Scientific, but am not sure...

12 July 2016 2,307 4 View

Anyone know a local stain or marking usable in rat heart tissue that can be seen during sectioning?

Hello - I am working on a project when I am doing a LAD ligation in a Rat heart and looking for a stain I can use to mark the spot and distinguish it during sectioning on the cryostat. Anyone have...

12 July 2016 3,833 1 View